Optical-fiber laser stimulation at 637, 594 or 473 nm in mouse cortex without e…

Optical-fiber laser stimulation at 637, 594 or 473 nm in mouse cortex without exogenous opsins evoked strong contralateral visual-cortex neuronal responses even at low intensities (1 mW at fiber tip), most pronounced at 637 nm — a methodological caveat for optogenetic-stimulation studies in mouse cortex.

{
  "n": 0,
  "doi": "10.1016/j.isci.2024.111152",
  "claim": "Optical-fiber laser stimulation at 637, 594 or 473 nm in mouse cortex without exogenous opsins evoked strong contralateral visual-cortex neuronal responses even at low intensities (1 mW at fiber tip), most pronounced at 637 nm — a methodological caveat for optogenetic-stimulation studies in mouse cortex.",
  "cite_key": "Weiler2024",
  "evidence": "Opsin-free control mice; cross-wavelength dose response; dim background-light rescue suppresses off-target activation at 594/473 nm but not at higher 637-nm intensities.",
  "effect_size": "responses at 1 mW fiber tip power; ≤2.5 mW required to suppress 637-nm-evoked off-target activation under dim light",
  "text_access": "fulltext",
  "study_system": "Mouse cortex in vivo, opsin-free controls",
  "argument_role": "supporting",
  "replication_status": "single-study-methodological-caveat",
  "claim_source_sentence": "Here, we found that optical fiber laser stimulation at wavelengths of 637, 594, or 473 nm within the cortex of mice lacking expression of exogenous opsins resulted in a strong neuronal response in the contralateral visual cortex. Evoked responses were observed even at low laser intensities (fiber tip power 1 mW) and most pronounced at 637 nm.",
  "source_provenance_status": "ok",
  "replication_evidence_dois": [],
  "effect_size_source_sentence": "Evoked responses were observed even at low laser intensities (fiber tip power 1 mW) and most pronounced at 637 nm."
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Here, we found that optical fiber laser stimulation at wavelengths of 637, 594, or 473 nm within the cortex of mice lacking expression of exogenous opsins resulted in a strong neuronal response in the contralateral visual cortex. Evoked responses were observed even at low laser intensities (fiber tip power 1 mW) and most pronounced at 637 nm.

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15. Methodological limits and emerging tools — what current mouse-cortex tools cannot yet measure about E→E recurrence (subthreshold network activity, fast plasticity in vivo, millimetre-scale dynamic connectomes), and what is on the near horizon

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