Two-photon optogenetic mapping to L2/3 pyramidal cells in mouse V1 measures L4→…

Two-photon optogenetic mapping to L2/3 pyramidal cells in mouse V1 measures L4→L2/3 connection probabilities of ~7-11% (Rorb-Cre AAV 7.8%, Rorb-Cre Ai167 10.7%, Scnn1a-Cre Ai167 9.1%) within 300 µm horizontal distance and identifies L5 IT (Rorb/Tlx3) translaminar connections only one-third weaker than L4 inputs (L5 6.9% vs L4 9.8%).

{
  "n": ">2500 probed potential connections across Cre lines; n in main text",
  "doi": "10.7554/elife.71103",
  "claim": "Two-photon optogenetic mapping to L2/3 pyramidal cells in mouse V1 measures L4→L2/3 connection probabilities of ~7-11% (Rorb-Cre AAV 7.8%, Rorb-Cre Ai167 10.7%, Scnn1a-Cre Ai167 9.1%) within 300 µm horizontal distance and identifies L5 IT (Rorb/Tlx3) translaminar connections only one-third weaker than L4 inputs (L5 6.9% vs L4 9.8%).",
  "cite_key": "Hage2022",
  "evidence": "Spatially precise two-photon optogenetic activation of single Cre-defined presynaptic neurons combined with paired/multicellular recordings of L2/3 PCs in mouse V1.",
  "effect_size": "L4→L2/3 P~7.8-10.7%; L5 IT→L2/3 P=6.9%; differences statistically robust (Fisher p=0.01 for L4 vs L5 IT)",
  "text_access": "fulltext",
  "study_system": "mouse V1; two-photon optogenetics + multicellular ephys",
  "argument_role": "supporting",
  "replication_status": "consistent with paired-recording estimates of L4→L2/3 in barrel and V1 (Petersen & Crochet; Cossell 2015)",
  "claim_source_sentence": "Rorb:Ai167: 10.7%, 110 connections/1022 probed, Rorb:AAV: 7.8%, 38 connections/489 probed, Scnn1a:Ai167: 9.1%, 12 connections/132 probed). ... connection probability from Rorb neurons located in L5 was only one-third lower than the connectivity of Rorb neurons in L4 (; combining Ai167 and AAV data within 300 µm horizontal distance, Rorb L4: 9.8%, 148 connections/1511 probed, Rorb L5: 6.9%, 71 connections /1028 probed, 6.9%, Fisher’s exact p=0.01).",
  "source_provenance_status": "ok",
  "replication_evidence_dois": [
    "10.1038/nature14182"
  ],
  "effect_size_source_sentence": "Rorb L4: 9.8%, 148 connections/1511 probed, Rorb L5: 6.9%, 71 connections /1028 probed, 6.9%, Fisher's exact p=0.01."
}

[
  "paper:paper-14e5fb681943"
]

Rorb:Ai167: 10.7%, 110 connections/1022 probed, Rorb:AAV: 7.8%, 38 connections/489 probed, Scnn1a:Ai167: 9.1%, 12 connections/132 probed). ... connection probability from Rorb neurons located in L5 was only one-third lower than the connectivity of Rorb neurons in L4 (; combining Ai167 and AAV data within 300 µm horizontal distance, Rorb L4: 9.8%, 148 connections/1511 probed, Rorb L5: 6.9%, 71 connections /1028 probe...

[
  {
    "ref": "paper:paper-14e5fb681943"
  }
]

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  "notes": [
    "Local review repositories are read-only inputs.",
    "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
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  "source_commit_sha": "79ce062d54a924ce05953ec90aa9d26044d2b48f",
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