Brain-wide light-sheet imaging and manual reconstruction of 1,000+ individual p…

Brain-wide light-sheet imaging and manual reconstruction of 1,000+ individual projection neurons in mouse motor cortex, thalamus, subiculum and hypothalamus identifies previously undescribed projection-neuron subtypes and reveals organizational principles of long-range corticocortical and cortico-subcortical wiring (e.g. discrete IT, PT, CT subtype axonal patterns within mouse M1 L5).

{
  "n": 1000,
  "doi": "10.1016/j.cell.2019.07.042",
  "claim": "Brain-wide light-sheet imaging and manual reconstruction of 1,000+ individual projection neurons in mouse motor cortex, thalamus, subiculum and hypothalamus identifies previously undescribed projection-neuron subtypes and reveals organizational principles of long-range corticocortical and cortico-subcortical wiring (e.g. discrete IT, PT, CT subtype axonal patterns within mouse M1 L5).",
  "cite_key": "Winnubst2019",
  "evidence": "High-throughput serial-block-face two-photon imaging plus manual single-neuron tracing of mouse cortical and subcortical projection neurons.",
  "effect_size": "quantitative — >1,000 reconstructed neurons across 4 brain regions",
  "text_access": "fulltext",
  "study_system": "mouse motor cortex, thalamus, subiculum, hypothalamus projection neurons",
  "argument_role": "supporting",
  "replication_status": "independently_replicated",
  "claim_source_sentence": "we reconstructed more than 1,000 projection neurons in the motor cortex, thalamus, subiculum, and hypothalamus. Together, the reconstructed neurons comprise more than 85 meters of axonal length and are available in a searchable online database. Axonal shapes revealed previously unknown subtypes of projection neurons and suggest organizational principles of long-range connectivity",
  "source_provenance_status": "ok",
  "replication_evidence_dois": [
    "10.1038/s41586-021-04020-1"
  ],
  "effect_size_source_sentence": "more than 1,000 projection neurons in the motor cortex, thalamus, subiculum, and hypothalamus"
}

[
  "paper:041efce6-fc13-4416-8aca-e196f82f3d70"
]

we reconstructed more than 1,000 projection neurons in the motor cortex, thalamus, subiculum, and hypothalamus. Together, the reconstructed neurons comprise more than 85 meters of axonal length and are available in a searchable online database. Axonal shapes revealed previously unknown subtypes of projection neurons and suggest organizational principles of long-range connectivity

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    "Local review repositories are read-only inputs.",
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  "source_commit_sha": "79ce062d54a924ce05953ec90aa9d26044d2b48f",
  "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence"
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