What resolves this contention: iPSC-derived astrocytes can be generated at high…

What resolves this contention: iPSC-derived astrocytes can be generated at high efficiency, express appropriate markers (S100β, GFAP), exhibit regional patterning, integrate with vasculature in vivo, and recapitulate disease-relevant phenotypes such as elevated Cx43 in ALS vs. iPSC-derived astrocytes and cortical organoid astrocytes transcriptionally correspond to mid-fetal developmental stages (19–24 post-conception weeks) and may not fully mature to adult human astrocyte states, raising concerns about modeling late-onset diseases / Human pluripotent stem cells can be directed to nearly uniform populations of immature astrocytes expressing S100β and GFAP at high efficiency. / Human cortical spheroids (hCSs) generated from pluripotent stem cells contain both deep and superficial cortical layer neurons and transcriptionally map to mid-fetal prenatal brain development (19–24 post-conception weeks).