High-resolution analysis of DNA regulatory elements by synthetic saturation mutagenesis

Nature Biotechnology 2009 411 cites
2009:132010:182011:232012:282013:332014:362015:402016:422017:442018:452019:452020:44
2009 2020
411

Rupali P Patwardhan, Choli Lee, Oren Litvin, David Young, Dana Pe’er et al.

Abstract

We present a method that harnesses massively parallel DNA synthesis and sequencing for the high-throughput functional analysis of regulatory sequences at single-nucleotide resolution. As a proof of concept, we quantitatively assayed the effects of all possible single-nucleotide mutations for three bacteriophage promoters and three mammalian core promoters in a single experiment per promoter. The method may also serve as a rapid screening tool for regulatory element engineering in synthetic biology.

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