{
"axis": {
"x": "Model system",
"y": "Measured parameter"
},
"notes": "Human astrocytes retain their species-specific morphological and functional identity (large size, fast Ca²⁺ signaling) when engrafted into mouse brain or grown as organoids, validating chimeric and organoid platforms for studying human-specific astrocyte biology in disease.",
"theme": "Human astrocyte identity preservation in chimeric mouse models",
"papers": [
"10.1016/j.stem.2012.12.015",
"10.1038/nbt.1877",
"10.1038/nmeth.3415",
"10.1186/s13024-021-00487-8"
],
"datapoints": [
{
"doi": "10.1016/j.stem.2012.12.015",
"group": "Engrafted human astrocyte process span (chimeric mouse) / Engrafted human Ca²⁺ wave speed (chimeric mouse) / Host murine Ca²⁺ wave speed (chimeric mouse)",
"units": "mm (minimum)",
"value": 0.5,
"values": {
"Host murine Ca²⁺ wave speed (chimeric mouse)": 5.7,
"Engrafted human Ca²⁺ wave speed (chimeric mouse)": 15.8,
"Engrafted human astrocyte process span (chimeric mouse)": 0.5
},
"source_sentence": "Line scanning with high temporal resolution (2-4 ms) showed that intracellular Ca 2+ wave propagation was significantly faster in human astrocytes than murine cells; intracellular Ca 2+ increases propagated with a velocity of 15.8 ± 0.7 μm/s among human glia, compared to 5.7 ± 0.4 μm/s in resident murine astrocytes (n=22-34, 6.",
"value_source_sentence": "Line scanning with high temporal resolution (2-4 ms) showed that intracellular Ca 2+ wave propagation was significantly faster in human astrocytes than murine cells; intracellular Ca 2+ increases propagated with a velocity of 15.8 ± 0.7 μm/s among human glia, compared to 5.7 ± 0.4 μm/s in resident murine astrocytes (n=22-34, 6."
},
{
"doi": "10.1038/nbt.1877",
"group": "hPSC-derived astrocyte differentiation efficiency (S100β+)",
"units": "% (minimum)",
"value": 90,
"values": {
"hPSC-derived astrocyte differentiation efficiency (S100β+)": 90
},
"source_sentence": "In this study, hPSCs were directed to nearly uniform populations of immature astrocytes (>90% S100β(+) and GFAP(+)) in large quantities.",
"value_source_sentence": "In this study, hPSCs were directed to nearly uniform populations of immature astrocytes (>90% S100β(+) and GFAP(+)) in large quantities."
},
{
"doi": "10.1038/nmeth.3415",
"group": "hCS organoid – astrocyte emergence time",
"units": "weeks in vitro",
"value": 7,
"values": {
"hCS organoid – astrocyte emergence time": 7
},
"source_sentence": "However, after ~7 weeks of differentiation in vitro , we observed astrocytes with thin GFAP + processes intermingled with NEUN + cells in the hCS parenchyma ( Fig.",
"value_source_sentence": "However, after ~7 weeks of differentiation in vitro , we observed astrocytes with thin GFAP + processes intermingled with NEUN + cells in the hCS parenchyma ( Fig."
},
{
"doi": "10.1186/s13024-021-00487-8",
"group": "Transplanted hiPSC astrocytes – cells recorded (WT chimera)",
"units": "cells from 6 mice",
"value": 17,
"values": {
"Transplanted hiPSC astrocytes – cells recorded (WT chimera)": 17
},
"source_sentence": "Representative traces of current injection steps of 20mV ( i ), resting membrane potentials ( j ) and current-voltage (I/V) curves ( k ) of hiPSC-astrocytes in the host brain (n = 17 cells from 6 WT mice).",
"value_source_sentence": "Representative traces of current injection steps of 20mV ( i ), resting membrane potentials ( j ) and current-voltage (I/V) curves ( k ) of hiPSC-astrocytes in the host brain (n = 17 cells from 6 WT mice)."
}
],
"comparison_id": "chimeric_human_astrocyte_fidelity"
}