Version history

{
  "kind": "infographic",
  "prompt": "Species Differences and Human Disease figure 5",
  "provider": "other",
  "raw_fields": {
    "axis": {
      "x": "Model system",
      "y": "Measured parameter"
    },
    "notes": "Human astrocytes retain their species-specific morphological and functional identity (large size, fast Ca²⁺ signaling) when engrafted into mouse brain or grown as organoids, validating chimeric and organoid platforms for studying human-specific astrocyte biology in disease.",
    "theme": "Human astrocyte identity preservation in chimeric mouse models",
    "papers": [
      "10.1016/j.stem.2012.12.015",
      "10.1038/nbt.1877",
      "10.1038/nmeth.3415",
      "10.1186/s13024-021-00487-8"
    ],
    "datapoints": [
      {
        "doi": "10.1016/j.stem.2012.12.015",
        "group": "Engrafted human astrocyte process span (chimeric mouse) / Engrafted human Ca²⁺ wave speed (chimeric mouse) / Host murine Ca²⁺ wave speed (chimeric mouse)",
        "units": "mm (minimum)",
        "value": 0.5,
        "values": {
          "Host murine Ca²⁺ wave speed (chimeric mouse)": 5.7,
          "Engrafted human Ca²⁺ wave speed (chimeric mouse)": 15.8,
          "Engrafted human astrocyte process span (chimeric mouse)": 0.5
        },
        "source_sentence": "Line scanning with high temporal resolution (2-4 ms) showed that intracellular Ca 2+ wave propagation was significantly faster in human astrocytes than murine cells; intracellular Ca 2+ increases propagated with a velocity of 15.8 ± 0.7 μm/s among human glia, compared to 5.7 ± 0.4 μm/s in resident murine astrocytes (n=22-34, 6.",
        "value_source_sentence": "Line scanning with high temporal resolution (2-4 ms) showed that intracellular Ca 2+ wave propagation was significantly faster in human astrocytes than murine cells; intracellular Ca 2+ increases propagated with a velocity of 15.8 ± 0.7 μm/s among human glia, compared to 5.7 ± 0.4 μm/s in resident murine astrocytes (n=22-34, 6."
      },
      {
        "doi": "10.1038/nbt.1877",
        "group": "hPSC-derived astrocyte differentiation efficiency (S100β+)",
        "units": "% (minimum)",
        "value": 90,
        "values": {
          "hPSC-derived astrocyte differentiation efficiency (S100β+)": 90
        },
        "source_sentence": "In this study, hPSCs were directed to nearly uniform populations of immature astrocytes (>90% S100β(+) and GFAP(+)) in large quantities.",
        "value_source_sentence": "In this study, hPSCs were directed to nearly uniform populations of immature astrocytes (>90% S100β(+) and GFAP(+)) in large quantities."
      },
      {
        "doi": "10.1038/nmeth.3415",
        "group": "hCS organoid – astrocyte emergence time",
        "units": "weeks in vitro",
        "value": 7,
        "values": {
          "hCS organoid – astrocyte emergence time": 7
        },
        "source_sentence": "However, after ~7 weeks of differentiation in vitro , we observed astrocytes with thin GFAP + processes intermingled with NEUN + cells in the hCS parenchyma ( Fig.",
        "value_source_sentence": "However, after ~7 weeks of differentiation in vitro , we observed astrocytes with thin GFAP + processes intermingled with NEUN + cells in the hCS parenchyma ( Fig."
      },
      {
        "doi": "10.1186/s13024-021-00487-8",
        "group": "Transplanted hiPSC astrocytes – cells recorded (WT chimera)",
        "units": "cells from 6 mice",
        "value": 17,
        "values": {
          "Transplanted hiPSC astrocytes – cells recorded (WT chimera)": 17
        },
        "source_sentence": "Representative traces of current injection steps of 20mV ( i ), resting membrane potentials ( j ) and current-voltage (I/V) curves ( k ) of hiPSC-astrocytes in the host brain (n = 17 cells from 6 WT mice).",
        "value_source_sentence": "Representative traces of current injection steps of 20mV ( i ), resting membrane potentials ( j ) and current-voltage (I/V) curves ( k ) of hiPSC-astrocytes in the host brain (n = 17 cells from 6 WT mice)."
      }
    ],
    "comparison_id": "chimeric_human_astrocyte_fidelity"
  },
  "section_id": "section_12_evidence_package",
  "source_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewAstrocytes/blob/1a55da0634a3bc04e5688792ed12141ce271d28e/evidence/section_12_evidence_package.json",
  "target_ref": "wiki_page:computationalreviewastrocytes-12",
  "review_repo": "ComputationalReviewAstrocytes",
  "section_ref": "wiki_page:computationalreviewastrocytes-12",
  "source_path": "evidence/section_12_evidence_package.json",
  "source_refs": [
    "paper:paper-2d9a059ff4a2",
    "paper:paper-374a998f7856",
    "paper:paper-a9b076f3b9d0",
    "paper:paper-c0990b7b4903"
  ],
  "section_title": "Species Differences and Human Disease",
  "source_policy": {
    "mode": "public_source_pointer_with_short_context",
    "notes": [
      "Local review repositories are read-only inputs.",
      "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
    ],
    "source_commit_sha": "1a55da0634a3bc04e5688792ed12141ce271d28e",
    "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewAstrocytes"
  },
  "generation_status": "complete",
  "review_bundle_ref": "analysis_bundle:ab-029ee9411fe2",
  "origin_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewAstrocytes/blob/1a55da0634a3bc04e5688792ed12141ce271d28e/evidence/section_12_evidence_package.json",
  "commit_sha": "1a55da0634a3bc04e5688792ed12141ce271d28e",
  "created_by": "persona-jerome-lecoq-gbo-neuroscience",
  "repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewAstrocytes"
}