Cross-method concordance for brain receptor expression mapping

{
  "title": "Cross-method concordance for brain receptor expression mapping",
  "papers": [
    {
      "doi": "10.1038/s41593-022-01186-3",
      "value": "high correlation for all 5 serotonin targets; replicated in independent autoradiography dataset",
      "comparison": "PET vs autoradiography",
      "methods_compared": "PET binding potential vs postmortem autoradiography",
      "value_source_sentence": "We replicated all findings in an independently collected autoradiography dataset."
    },
    {
      "doi": "10.1523/jneurosci.2830-16.2016",
      "value": "strong PET-autoradiography correlation; receptor-specific PET-mRNA associations",
      "comparison": "PET vs autoradiography vs mRNA",
      "methods_compared": "PET vs autoradiography vs Allen Human Brain Atlas mRNA",
      "value_source_sentence": "Comparison of the regional PET binding measures with postmortem human brain autoradiography outcomes showed a high correlation for the five 5-HT targets and this enabled us to transform the atlas to represent protein densities (in picomoles per milliliter)."
    },
    {
      "doi": "10.1038/s41392-022-00960-w",
      "value": "seq-based lacks cellular resolution; image-based limited in transcriptome coverage",
      "comparison": "seq-based vs image-based spatial transcriptomics",
      "methods_compared": "sequencing-based ST vs FISH-based ST",
      "value_source_sentence": "Although current ST methods, whether based on next-generation sequencing (seq-based approaches) or fluorescence in situ hybridization (image-based approaches), offer valuable insights, they face limitations either in cellular resolution or transcriptome-wide profiling."
    }
  ],
  "x_axis": "Detection method",
  "y_axis": "Concordance metric",
  "n_analyzed": "varies",
  "n_definition": "varies: subjects (PET), tissue samples (autoradiography), genes (mRNA)",
  "scope_region": "whole brain",
  "comparison_id": "fig_sec3_method_concordance",
  "comparison_type": "methodological comparison",
  "taxonomic_level": "receptor/transporter level",
  "scope_population": "receptor expression measurements",
  "homogeneity_check": "Comparisons span different measurement modalities (protein vs mRNA vs binding), different species (human vs mouse), and different spatial resolutions. PET-autoradiography comparisons are protein-level but differ in spatial resolution and in vivo vs ex vivo conditions. mRNA-protein comparisons are fundamentally different molecular readouts. These methodological differences ARE the point of this comparison figure."
}

[
  "paper:paper-8c66fed0864e",
  "paper:paper-ac1398cc9f7b",
  "paper:paper-e1ce9847cb04"
]

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  "notes": [
    "Local review repositories are read-only inputs.",
    "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
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  "source_commit_sha": "95db5c630fe54e183d9c452cf826ce502d4a872d",
  "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewNeuromodulation"
}