Version history

{
  "kind": "infographic",
  "prompt": "Cross-method concordance for brain receptor expression mapping",
  "provider": "other",
  "raw_fields": {
    "title": "Cross-method concordance for brain receptor expression mapping",
    "papers": [
      {
        "doi": "10.1038/s41593-022-01186-3",
        "value": "high correlation for all 5 serotonin targets; replicated in independent autoradiography dataset",
        "comparison": "PET vs autoradiography",
        "methods_compared": "PET binding potential vs postmortem autoradiography",
        "value_source_sentence": "We replicated all findings in an independently collected autoradiography dataset."
      },
      {
        "doi": "10.1523/jneurosci.2830-16.2016",
        "value": "strong PET-autoradiography correlation; receptor-specific PET-mRNA associations",
        "comparison": "PET vs autoradiography vs mRNA",
        "methods_compared": "PET vs autoradiography vs Allen Human Brain Atlas mRNA",
        "value_source_sentence": "Comparison of the regional PET binding measures with postmortem human brain autoradiography outcomes showed a high correlation for the five 5-HT targets and this enabled us to transform the atlas to represent protein densities (in picomoles per milliliter)."
      },
      {
        "doi": "10.1038/s41392-022-00960-w",
        "value": "seq-based lacks cellular resolution; image-based limited in transcriptome coverage",
        "comparison": "seq-based vs image-based spatial transcriptomics",
        "methods_compared": "sequencing-based ST vs FISH-based ST",
        "value_source_sentence": "Although current ST methods, whether based on next-generation sequencing (seq-based approaches) or fluorescence in situ hybridization (image-based approaches), offer valuable insights, they face limitations either in cellular resolution or transcriptome-wide profiling."
      }
    ],
    "x_axis": "Detection method",
    "y_axis": "Concordance metric",
    "n_analyzed": "varies",
    "n_definition": "varies: subjects (PET), tissue samples (autoradiography), genes (mRNA)",
    "scope_region": "whole brain",
    "comparison_id": "fig_sec3_method_concordance",
    "comparison_type": "methodological comparison",
    "taxonomic_level": "receptor/transporter level",
    "scope_population": "receptor expression measurements",
    "homogeneity_check": "Comparisons span different measurement modalities (protein vs mRNA vs binding), different species (human vs mouse), and different spatial resolutions. PET-autoradiography comparisons are protein-level but differ in spatial resolution and in vivo vs ex vivo conditions. mRNA-protein comparisons are fundamentally different molecular readouts. These methodological differences ARE the point of this comparison figure."
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  "source_refs": [
    "paper:paper-8c66fed0864e",
    "paper:paper-ac1398cc9f7b",
    "paper:paper-e1ce9847cb04"
  ],
  "section_title": "Receptor Pharmacology and Region-Specific Expression",
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      "Local review repositories are read-only inputs.",
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}