{
"papers": [
{
"n": 61,
"doi": "10.1002/glia.20991",
"value": "80%",
"method": "dye coupling in KO vs WT",
"metric": "Percent reduction in coupled-cell count vs WT",
"n_analyzed": "",
"ci_or_error": "",
"text_access": "abstract_only",
"n_definition": "injected cells",
"scope_region": "varies",
"study_system": "astrocyte network in KO mice vs WT",
"taxonomic_level": "network-level % reduction",
"scope_population": "astrocytes or panglial network",
"value_source_sentence": "In Cx47-deficient mice, the number of coupled cells was reduced by 80%.",
"experimental_conditions": "genetic deletion of Cx43 and/or Cx30 (or Cx47)"
},
{
"n": 0,
"doi": "10.3390/biom10111578",
"value": "abolished",
"method": "dye coupling in KO vs WT",
"metric": "coupling effect of genetic manipulation",
"n_analyzed": "",
"ci_or_error": "",
"text_access": "fulltext",
"n_definition": "injected cells",
"scope_region": "varies",
"study_system": "astrocyte network in KO mice",
"taxonomic_level": "network-level effect",
"scope_population": "astrocytes or panglial network",
"value_source_sentence": "However, coupling was not completely abolished due to compensatory changes of Cx30 [ 124 , 125 , 126 ].",
"experimental_conditions": "genetic deletion of Cx43/Cx30/Cx47"
},
{
"n": 6,
"doi": "10.1371/journal.pgen.1002146",
"value": "88%",
"method": "dye coupling in KO vs WT",
"metric": "Percent reduction in coupled-cell count vs WT",
"n_analyzed": "",
"ci_or_error": "",
"text_access": "fulltext",
"n_definition": "injected cells",
"scope_region": "varies",
"study_system": "astrocyte network in KO mice vs WT",
"taxonomic_level": "network-level % reduction",
"scope_population": "astrocytes or panglial network",
"value_source_sentence": "Accordingly, biocytin injections into corpus callosum oligodendrocytes participating in glial networks revealed a 88% decrease in number of coupled cells in Cx47 M282T/M282T P10–P15 mice which was very similar to the 85% reduction observed with Cx47 −/− mice.",
"experimental_conditions": "genetic deletion of Cx43 and/or Cx30 (or Cx47)"
},
{
"n": 0,
"doi": "10.1016/j.bbamem.2011.08.015",
"value": "50%",
"method": "dye coupling in KO vs WT",
"metric": "Percent reduction in coupled-cell count vs WT",
"n_analyzed": "",
"ci_or_error": "",
"text_access": "fulltext",
"n_definition": "injected cells",
"scope_region": "varies",
"study_system": "astrocyte network in KO mice vs WT",
"taxonomic_level": "network-level % reduction",
"scope_population": "astrocytes or panglial network",
"value_source_sentence": "Both Wallraff et al. [ 13 ] and Theis et al. [ 42 ] studied mice with astrocyte specific deletion of Cx43 alone and found approximately a 50% reduction in A/A coupling.",
"experimental_conditions": "genetic deletion of Cx43 and/or Cx30 (or Cx47)"
},
{
"n": 0,
"doi": "10.1016/j.bbamem.2012.01.024",
"value": "80%",
"method": "dye coupling in KO vs WT",
"metric": "Percent reduction in coupled-cell count vs WT",
"n_analyzed": "",
"ci_or_error": "",
"text_access": "fulltext",
"n_definition": "injected cells",
"scope_region": "varies",
"study_system": "astrocyte network in KO mice vs WT",
"taxonomic_level": "network-level % reduction",
"scope_population": "astrocytes or panglial network",
"value_source_sentence": "Maglione et al., (2010) [ 56 ] report that the number of oligodendrocytes coupled to other oligodendrocytes in white matter is reduced 80% in Cx47 KO mice.",
"experimental_conditions": "genetic deletion of Cx43 and/or Cx30 (or Cx47)"
},
{
"n": 30,
"doi": "10.3389/fncel.2017.00174",
"value": "abolished",
"method": "dye coupling in KO vs WT",
"metric": "coupling effect of genetic manipulation",
"n_analyzed": "",
"ci_or_error": "",
"text_access": "fulltext",
"n_definition": "injected cells",
"scope_region": "varies",
"study_system": "astrocyte network in KO mice",
"taxonomic_level": "network-level effect",
"scope_population": "astrocytes or panglial network",
"value_source_sentence": "Coupling between astrocytes is greatly reduced in cells lacking Cx43 and abolished when both Cx43 and Cx30 are absent (Perez Velazquez et al., 1996 ; Naus et al., 1997 ; Wallraff et al., 2006 ; Rouach et al., 2008 ; Roux et al., 2011 ), demonstrating that these two Cxs are the main molecular support for direct intercellular communication in astrocytes.",
"experimental_conditions": "genetic deletion of Cx43/Cx30/Cx47"
},
{
"n": 56,
"doi": "10.3390/biom12121812",
"value": "abolished",
"method": "dye coupling in KO vs WT",
"metric": "coupling effect of genetic manipulation",
"n_analyzed": "",
"ci_or_error": "",
"text_access": "fulltext",
"n_definition": "injected cells",
"scope_region": "varies",
"study_system": "astrocyte network in KO mice",
"taxonomic_level": "network-level effect",
"scope_population": "astrocytes or panglial network",
"value_source_sentence": "Consequently, cell-to-cell coupling for small ions and molecules is strongly reduced in Cx43 KO [ 58 ] and abolished in double-knockout mice lacking both Cx43 and Cx30 [ 32 , 59 , 61 ].",
"experimental_conditions": "genetic deletion of Cx43/Cx30/Cx47"
}
],
"comparison_id": "connexin_KO_coupling_reduction",
"comparison_name": "Effect of genetic connexin deletion on astrocyte/panglial coupling",
"comparison_type": "convergent evidence",
"what_it_reveals": "Loss of individual connexins produces graded coupling reductions (≈50% with Cx43 alone, ≈80% with Cx47, ≈100% with Cx43/Cx30 DKO); connexin identity and stoichiometry govern the residual coupling.",
"homogeneity_check": {
"caveats": [
"Entries span single-KO, double-KO, and point-mutant studies.",
"Some values are from review papers citing primary work (10.1016/j.bbamem.2011.08.015 cites Wallraff & Theis).",
"Some entries are panglial (Cx47 KO affecting OL–astro coupling) not purely astrocytic."
],
"n_definition_uniform": "false",
"scope_region_uniform": "false",
"taxonomic_level_uniform": "true",
"scope_population_uniform": "false"
},
"suggested_plot_type": "forest plot"
}