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{ "kind": "infographic", "prompt": "Loss of individual connexins produces graded coupling reductions (≈50% with Cx43 alone, ≈80% with Cx47, ≈100% with Cx43/Cx30 DKO); connexin identity and stoichiometry govern the residual coupling.", "provider": "other", "raw_fields": { "papers": [ { "n": 61, "doi": "10.1002/glia.20991", "value": "80%", "method": "dye coupling in KO vs WT", "metric": "Percent reduction in coupled-cell count vs WT", "n_analyzed": "", "ci_or_error": "", "text_access": "abstract_only", "n_definition": "injected cells", "scope_region": "varies", "study_system": "astrocyte network in KO mice vs WT", "taxonomic_level": "network-level % reduction", "scope_population": "astrocytes or panglial network", "value_source_sentence": "In Cx47-deficient mice, the number of coupled cells was reduced by 80%.", "experimental_conditions": "genetic deletion of Cx43 and/or Cx30 (or Cx47)" }, { "n": 0, "doi": "10.3390/biom10111578", "value": "abolished", "method": "dye coupling in KO vs WT", "metric": "coupling effect of genetic manipulation", "n_analyzed": "", "ci_or_error": "", "text_access": "fulltext", "n_definition": "injected cells", "scope_region": "varies", "study_system": "astrocyte network in KO mice", "taxonomic_level": "network-level effect", "scope_population": "astrocytes or panglial network", "value_source_sentence": "However, coupling was not completely abolished due to compensatory changes of Cx30 [ 124 , 125 , 126 ].", "experimental_conditions": "genetic deletion of Cx43/Cx30/Cx47" }, { "n": 6, "doi": "10.1371/journal.pgen.1002146", "value": "88%", "method": "dye coupling in KO vs WT", "metric": "Percent reduction in coupled-cell count vs WT", "n_analyzed": "", "ci_or_error": "", "text_access": "fulltext", "n_definition": "injected cells", "scope_region": "varies", "study_system": "astrocyte network in KO mice vs WT", "taxonomic_level": "network-level % reduction", "scope_population": "astrocytes or panglial network", "value_source_sentence": "Accordingly, biocytin injections into corpus callosum oligodendrocytes participating in glial networks revealed a 88% decrease in number of coupled cells in Cx47 M282T/M282T P10–P15 mice which was very similar to the 85% reduction observed with Cx47 −/− mice.", "experimental_conditions": "genetic deletion of Cx43 and/or Cx30 (or Cx47)" }, { "n": 0, "doi": "10.1016/j.bbamem.2011.08.015", "value": "50%", "method": "dye coupling in KO vs WT", "metric": "Percent reduction in coupled-cell count vs WT", "n_analyzed": "", "ci_or_error": "", "text_access": "fulltext", "n_definition": "injected cells", "scope_region": "varies", "study_system": "astrocyte network in KO mice vs WT", "taxonomic_level": "network-level % reduction", "scope_population": "astrocytes or panglial network", "value_source_sentence": "Both Wallraff et al. [ 13 ] and Theis et al. [ 42 ] studied mice with astrocyte specific deletion of Cx43 alone and found approximately a 50% reduction in A/A coupling.", "experimental_conditions": "genetic deletion of Cx43 and/or Cx30 (or Cx47)" }, { "n": 0, "doi": "10.1016/j.bbamem.2012.01.024", "value": "80%", "method": "dye coupling in KO vs WT", "metric": "Percent reduction in coupled-cell count vs WT", "n_analyzed": "", "ci_or_error": "", "text_access": "fulltext", "n_definition": "injected cells", "scope_region": "varies", "study_system": "astrocyte network in KO mice vs WT", "taxonomic_level": "network-level % reduction", "scope_population": "astrocytes or panglial network", "value_source_sentence": "Maglione et al., (2010) [ 56 ] report that the number of oligodendrocytes coupled to other oligodendrocytes in white matter is reduced 80% in Cx47 KO mice.", "experimental_conditions": "genetic deletion of Cx43 and/or Cx30 (or Cx47)" }, { "n": 30, "doi": "10.3389/fncel.2017.00174", "value": "abolished", "method": "dye coupling in KO vs WT", "metric": "coupling effect of genetic manipulation", "n_analyzed": "", "ci_or_error": "", "text_access": "fulltext", "n_definition": "injected cells", "scope_region": "varies", "study_system": "astrocyte network in KO mice", "taxonomic_level": "network-level effect", "scope_population": "astrocytes or panglial network", "value_source_sentence": "Coupling between astrocytes is greatly reduced in cells lacking Cx43 and abolished when both Cx43 and Cx30 are absent (Perez Velazquez et al., 1996 ; Naus et al., 1997 ; Wallraff et al., 2006 ; Rouach et al., 2008 ; Roux et al., 2011 ), demonstrating that these two Cxs are the main molecular support for direct intercellular communication in astrocytes.", "experimental_conditions": "genetic deletion of Cx43/Cx30/Cx47" }, { "n": 56, "doi": "10.3390/biom12121812", "value": "abolished", "method": "dye coupling in KO vs WT", "metric": "coupling effect of genetic manipulation", "n_analyzed": "", "ci_or_error": "", "text_access": "fulltext", "n_definition": "injected cells", "scope_region": "varies", "study_system": "astrocyte network in KO mice", "taxonomic_level": "network-level effect", "scope_population": "astrocytes or panglial network", "value_source_sentence": "Consequently, cell-to-cell coupling for small ions and molecules is strongly reduced in Cx43 KO [ 58 ] and abolished in double-knockout mice lacking both Cx43 and Cx30 [ 32 , 59 , 61 ].", "experimental_conditions": "genetic deletion of Cx43/Cx30/Cx47" } ], "comparison_id": "connexin_KO_coupling_reduction", "comparison_name": "Effect of genetic connexin deletion on astrocyte/panglial coupling", "comparison_type": "convergent evidence", "what_it_reveals": "Loss of individual connexins produces graded coupling reductions (≈50% with Cx43 alone, ≈80% with Cx47, ≈100% with Cx43/Cx30 DKO); connexin identity and stoichiometry govern the residual coupling.", "homogeneity_check": { "caveats": [ "Entries span single-KO, double-KO, and point-mutant studies.", "Some values are from review papers citing primary work (10.1016/j.bbamem.2011.08.015 cites Wallraff & Theis).", "Some entries are panglial (Cx47 KO affecting OL–astro coupling) not purely astrocytic." ], "n_definition_uniform": "false", "scope_region_uniform": "false", "taxonomic_level_uniform": "true", "scope_population_uniform": "false" }, "suggested_plot_type": "forest plot" }, "section_id": "section_07_evidence_package", "source_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewAstrocytes/blob/1a55da0634a3bc04e5688792ed12141ce271d28e/evidence/section_07_evidence_package.json", "target_ref": "wiki_page:computationalreviewastrocytes-07", "review_repo": "ComputationalReviewAstrocytes", "section_ref": "wiki_page:computationalreviewastrocytes-07", "source_path": "evidence/section_07_evidence_package.json", "source_refs": [ "paper:paper-3bd2a5e5d559", "paper:paper-49069969d2f7", "paper:paper-a173439cede0", "paper:paper-a53f3baa1b97", "paper:paper-b3cf48996b01", "paper:paper-c21044ddc7df", "paper:paper-dfd928fac4a2" ], "section_title": "Gap-Junctional Coupling and Astrocyte Networks", "source_policy": { "mode": "public_source_pointer_with_short_context", "notes": [ "Local review repositories are read-only inputs.", "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose." ], "source_commit_sha": "1a55da0634a3bc04e5688792ed12141ce271d28e", "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewAstrocytes" }, "generation_status": "complete", "review_bundle_ref": "analysis_bundle:ab-029ee9411fe2", "origin_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewAstrocytes/blob/1a55da0634a3bc04e5688792ed12141ce271d28e/evidence/section_07_evidence_package.json", "commit_sha": "1a55da0634a3bc04e5688792ed12141ce271d28e", "created_by": "persona-jerome-lecoq-gbo-neuroscience", "repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewAstrocytes" }