{
"papers": [
{
"doi": "10.1371/journal.pone.0181113",
"note": "Ye et al 2017: GCaMP6f has faster kinetics than GCaMP3 but similar peak amplitude in awake-mouse astrocytes",
"value": "GCaMP6f: 35.2±2.0 %/s rise, 7.11±0.29 s time-to-peak; GCaMP3: 27.9±1.8 %/s rise, 8.58±0.45 s (p<0.05)",
"indicator": "GCaMP6f vs GCaMP3 (paired comparison)",
"measurements": [
{
"value": "rise rate 35.2 ± 2.0 %/s; time-to-peak 7.11 ± 0.29 s (n=8 mice)",
"indicator": "GCaMP6f",
"value_source_sentence": "Indeed, GCaMP6f fluorescent transients had a larger maximum rate of rise (35.2 ± 2.0% of peak / s; 8 mice) than GCaMP3 fluorescent transients (27.9 ± 1.8% of peak / s; 9 mice, p = 0.014), and there was a trend towards a faster maximum rate of fluorescence decay of GCaMP6f signals (-26.2 ± 2.1% of peak / s; 8 mice) compared to GCaMP3 (-22.3 ± 1.4% of peak / s; 9 mice, p = 0.094)."
},
{
"value": "rise rate 27.9 ± 1.8 %/s; time-to-peak 8.58 ± 0.45 s (n=9 mice)",
"indicator": "GCaMP3",
"value_source_sentence": "Consistent with these kinetic parameters, GCaMP6f signals reached the peak response sooner following onset of locomotion (7.11 ± 0.29 s; 8 mice) than GCaMP3 signals (8.58 ± 0.45 s; 9 mice, p = 0.017) ( Fig 1F and 1H )."
}
],
"value_source_sentence": "Indeed, GCaMP6f fluorescent transients had a larger maximum rate of rise (35.2 ± 2.0% of peak / s; 8 mice) than GCaMP3 fluorescent transients (27.9 ± 1.8% of peak / s; 9 mice, p = 0.014), and there was a trend towards a faster maximum rate of fluorescence decay of GCaMP6f signals (-26.2 ± 2.1% of peak / s; 8 mice) compared to GCaMP3 (-22.3 ± 1.4% of peak / s; 9 mice, p = 0.094)."
},
{
"doi": "10.1085/jgp.201210949",
"note": "Shigetomi 2013: membrane-targeted Lck-GCaMP exposes process microdomains invisible to cytosolic GCaMP",
"value": "cyto-GCaMP3 + Lck-GCaMP3 detect ~60–90 Ca2+ signals per astrocyte/5 min vs ~8 with Fluo-4AM (≈7–11× more)",
"indicator": "Lck-GCaMP3 / cyto-GCaMP3",
"value_source_sentence": "Both cyto-GCaMP3 and Lck-GCaMP3 were far superior to Fluo-4AM, and there was a trend for Lck-GCaMP3 to detect more Ca 2+ signals than cyto-GCaMP3 ( Fig. 8, B and C )."
},
{
"doi": "10.1002/glia.23042",
"note": "Agarwal 2017 / Rungta 2016 style paper: microdomain vs somatic kinetics",
"value": "Process Ca2+ transients dependent on Ca2+ influx (abolished by extracellular Ca2+ removal); intact in IP3R2-KO",
"indicator": "GCaMP6s in fine processes",
"value_source_sentence": "Removal of extracellular Ca<sup>2+</sup> almost completely and reversibly abolished the spontaneous signals while IP<sub>3</sub> R2 KO mice also exhibited spontaneous and compartmentalized signals, suggesting they rely on influx of extracellular Ca<sup>2+</sup>."
}
],
"axis_label": "Maximum rate of rise (% of peak / s) and time-to-peak (s)",
"comparison_topic": "Performance of GCaMP variants for astrocyte Ca2+ imaging (kinetic parameters, signal-to-noise)"
}