Version history

{
  "kind": "infographic",
  "prompt": "Astrocyte Calcium Signaling figure 3",
  "provider": "other",
  "raw_fields": {
    "papers": [
      {
        "doi": "10.1371/journal.pone.0181113",
        "note": "Ye et al 2017: GCaMP6f has faster kinetics than GCaMP3 but similar peak amplitude in awake-mouse astrocytes",
        "value": "GCaMP6f: 35.2±2.0 %/s rise, 7.11±0.29 s time-to-peak; GCaMP3: 27.9±1.8 %/s rise, 8.58±0.45 s (p<0.05)",
        "indicator": "GCaMP6f vs GCaMP3 (paired comparison)",
        "measurements": [
          {
            "value": "rise rate 35.2 ± 2.0 %/s; time-to-peak 7.11 ± 0.29 s (n=8 mice)",
            "indicator": "GCaMP6f",
            "value_source_sentence": "Indeed, GCaMP6f fluorescent transients had a larger maximum rate of rise (35.2 ± 2.0% of peak / s; 8 mice) than GCaMP3 fluorescent transients (27.9 ± 1.8% of peak / s; 9 mice, p = 0.014), and there was a trend towards a faster maximum rate of fluorescence decay of GCaMP6f signals (-26.2 ± 2.1% of peak / s; 8 mice) compared to GCaMP3 (-22.3 ± 1.4% of peak / s; 9 mice, p = 0.094)."
          },
          {
            "value": "rise rate 27.9 ± 1.8 %/s; time-to-peak 8.58 ± 0.45 s (n=9 mice)",
            "indicator": "GCaMP3",
            "value_source_sentence": "Consistent with these kinetic parameters, GCaMP6f signals reached the peak response sooner following onset of locomotion (7.11 ± 0.29 s; 8 mice) than GCaMP3 signals (8.58 ± 0.45 s; 9 mice, p = 0.017) ( Fig 1F and 1H )."
          }
        ],
        "value_source_sentence": "Indeed, GCaMP6f fluorescent transients had a larger maximum rate of rise (35.2 ± 2.0% of peak / s; 8 mice) than GCaMP3 fluorescent transients (27.9 ± 1.8% of peak / s; 9 mice, p = 0.014), and there was a trend towards a faster maximum rate of fluorescence decay of GCaMP6f signals (-26.2 ± 2.1% of peak / s; 8 mice) compared to GCaMP3 (-22.3 ± 1.4% of peak / s; 9 mice, p = 0.094)."
      },
      {
        "doi": "10.1085/jgp.201210949",
        "note": "Shigetomi 2013: membrane-targeted Lck-GCaMP exposes process microdomains invisible to cytosolic GCaMP",
        "value": "cyto-GCaMP3 + Lck-GCaMP3 detect ~60–90 Ca2+ signals per astrocyte/5 min vs ~8 with Fluo-4AM (≈7–11× more)",
        "indicator": "Lck-GCaMP3 / cyto-GCaMP3",
        "value_source_sentence": "Both cyto-GCaMP3 and Lck-GCaMP3 were far superior to Fluo-4AM, and there was a trend for Lck-GCaMP3 to detect more Ca 2+ signals than cyto-GCaMP3 ( Fig. 8, B and C )."
      },
      {
        "doi": "10.1002/glia.23042",
        "note": "Agarwal 2017 / Rungta 2016 style paper: microdomain vs somatic kinetics",
        "value": "Process Ca2+ transients dependent on Ca2+ influx (abolished by extracellular Ca2+ removal); intact in IP3R2-KO",
        "indicator": "GCaMP6s in fine processes",
        "value_source_sentence": "Removal of extracellular Ca<sup>2+</sup> almost completely and reversibly abolished the spontaneous signals while IP<sub>3</sub> R2 KO mice also exhibited spontaneous and compartmentalized signals, suggesting they rely on influx of extracellular Ca<sup>2+</sup>."
      }
    ],
    "axis_label": "Maximum rate of rise (% of peak / s) and time-to-peak (s)",
    "comparison_topic": "Performance of GCaMP variants for astrocyte Ca2+ imaging (kinetic parameters, signal-to-noise)"
  },
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    "paper:paper-b7b6019e29af",
    "paper:paper-c4ac0d62b042",
    "paper:paper-daae60fbce4a"
  ],
  "section_title": "Astrocyte Calcium Signaling",
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