Impact of Kv3 subunit knockouts on fast-spiking phenotype across studies

Progressive severity of FS phenotype disruption with increasing Kv3 gene loss: Kv3.1 single KO shows moderate 21% AP broadening with preserved FS core, Kv3.2 KO shows layer-specific impairment matching expression pattern, and Kv3.1/Kv3.2 DKO shows enhanced GABA release consistent with broadened presynaptic APs. This demonstrates both redundancy and complementarity within the Kv3 family.

{
  "papers": [
    {
      "n": 0,
      "doi": "10.1523/jneurosci.21-02-00485.2001",
      "value": "21",
      "method": "patch-clamp electrophysiology",
      "metric": "AP duration change in Kv3.1 KO (at 34°C)",
      "n_analyzed": "Kv3.1-/- vs WT RTN neurons",
      "ci_or_error": null,
      "text_access": "abstract_only",
      "n_definition": "reticular thalamic nucleus neurons recorded",
      "scope_region": "reticular thalamic nucleus",
      "study_system": "mouse RTN, Kv3.1 KO",
      "taxonomic_level": "cell type level",
      "scope_population": "FS neurons",
      "value_source_sentence": "action potentials in Kv3.1(-/-) mice had increased duration (21%) due to a reduced rate of repolarization (-30%) when compared with wild-type controls.",
      "experimental_conditions": "whole-cell current clamp at 34°C"
    },
    {
      "n": 0,
      "doi": "10.1523/jneurosci.20-24-09071.2000",
      "value": "impaired in deep layers (5-6), normal in superficial (2-4)",
      "method": "patch-clamp electrophysiology + immunocytochemistry",
      "metric": "Impaired high-frequency firing in Kv3.2 KO",
      "n_analyzed": "Kv3.2-/- vs WT cortical neurons",
      "ci_or_error": null,
      "text_access": "abstract_only",
      "n_definition": "cortical FS interneurons recorded",
      "scope_region": "neocortex layers 2-6",
      "study_system": "mouse neocortex, Kv3.2 KO",
      "taxonomic_level": "cell type by layer",
      "scope_population": "FS interneurons",
      "value_source_sentence": "The ability to fire spikes at high frequencies was impaired in FS interneurons of deep cortical layers (5-6). No such impairment was found for FS neurons of superficial layers (2-4).",
      "experimental_conditions": "whole-cell recording with immunocytochemical identification"
    },
    {
      "n": 0,
      "doi": "10.1523/jneurosci.0722-05.2005",
      "value": "larger spike-evoked GABA release, lower PPR",
      "method": "patch-clamp electrophysiology",
      "metric": "GABA release change in Kv3.1/Kv3.2 DKO",
      "n_analyzed": "Kv3.1/Kv3.2 DKO vs WT",
      "ci_or_error": null,
      "text_access": "abstract_only",
      "n_definition": "L2/3 pyramidal cells receiving FS input",
      "scope_region": "neocortex layer II/III",
      "study_system": "mouse neocortex, Kv3.1/Kv3.2 double KO",
      "taxonomic_level": "cell type level",
      "scope_population": "FS cell synapses",
      "value_source_sentence": "in Kv3.1/Kv3.2 double knock-out mice, spike-evoked GABA release was larger, and the PPR was lower than in wild-type mice.",
      "experimental_conditions": "FS cell-to-pyramidal cell paired recordings"
    }
  ],
  "comparison_id": "kv3-knockout-phenotypes",
  "comparison_name": "Impact of Kv3 subunit knockouts on fast-spiking phenotype across studies",
  "comparison_type": "convergent evidence",
  "what_it_reveals": "Progressive severity of FS phenotype disruption with increasing Kv3 gene loss: Kv3.1 single KO shows moderate 21% AP broadening with preserved FS core, Kv3.2 KO shows layer-specific impairment matching expression pattern, and Kv3.1/Kv3.2 DKO shows enhanced GABA release consistent with broadened presynaptic APs. This demonstrates both redundancy and complementarity within the Kv3 family.",
  "homogeneity_check": {
    "caveats": [
      "Different brain regions (RTN vs neocortex)",
      "Different metrics (AP duration vs firing rate vs GABA release)",
      "Single vs double knockout comparisons",
      "Kv3.1 KO study in RTN, others in cortex"
    ],
    "n_definition_uniform": "false",
    "scope_region_uniform": "false",
    "taxonomic_level_uniform": "true",
    "scope_population_uniform": "true"
  },
  "suggested_plot_type": "grouped bar"
}

[
  "paper:paper-08e60421a6c7",
  "paper:paper-20a89635af94",
  "paper:paper-f2a5716f0156"
]

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    "Local review repositories are read-only inputs.",
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