Version history

{
  "kind": "infographic",
  "prompt": "Progressive severity of FS phenotype disruption with increasing Kv3 gene loss: Kv3.1 single KO shows moderate 21% AP broadening with preserved FS core, Kv3.2 KO shows layer-specific impairment matching expression pattern, and Kv3.1/Kv3.2 DKO shows enhanced GABA release consistent with broadened presynaptic APs. This demonstrates both redundancy and complementarity within the Kv3 family.",
  "provider": "other",
  "raw_fields": {
    "papers": [
      {
        "n": 0,
        "doi": "10.1523/jneurosci.21-02-00485.2001",
        "value": "21",
        "method": "patch-clamp electrophysiology",
        "metric": "AP duration change in Kv3.1 KO (at 34°C)",
        "n_analyzed": "Kv3.1-/- vs WT RTN neurons",
        "ci_or_error": null,
        "text_access": "abstract_only",
        "n_definition": "reticular thalamic nucleus neurons recorded",
        "scope_region": "reticular thalamic nucleus",
        "study_system": "mouse RTN, Kv3.1 KO",
        "taxonomic_level": "cell type level",
        "scope_population": "FS neurons",
        "value_source_sentence": "action potentials in Kv3.1(-/-) mice had increased duration (21%) due to a reduced rate of repolarization (-30%) when compared with wild-type controls.",
        "experimental_conditions": "whole-cell current clamp at 34°C"
      },
      {
        "n": 0,
        "doi": "10.1523/jneurosci.20-24-09071.2000",
        "value": "impaired in deep layers (5-6), normal in superficial (2-4)",
        "method": "patch-clamp electrophysiology + immunocytochemistry",
        "metric": "Impaired high-frequency firing in Kv3.2 KO",
        "n_analyzed": "Kv3.2-/- vs WT cortical neurons",
        "ci_or_error": null,
        "text_access": "abstract_only",
        "n_definition": "cortical FS interneurons recorded",
        "scope_region": "neocortex layers 2-6",
        "study_system": "mouse neocortex, Kv3.2 KO",
        "taxonomic_level": "cell type by layer",
        "scope_population": "FS interneurons",
        "value_source_sentence": "The ability to fire spikes at high frequencies was impaired in FS interneurons of deep cortical layers (5-6). No such impairment was found for FS neurons of superficial layers (2-4).",
        "experimental_conditions": "whole-cell recording with immunocytochemical identification"
      },
      {
        "n": 0,
        "doi": "10.1523/jneurosci.0722-05.2005",
        "value": "larger spike-evoked GABA release, lower PPR",
        "method": "patch-clamp electrophysiology",
        "metric": "GABA release change in Kv3.1/Kv3.2 DKO",
        "n_analyzed": "Kv3.1/Kv3.2 DKO vs WT",
        "ci_or_error": null,
        "text_access": "abstract_only",
        "n_definition": "L2/3 pyramidal cells receiving FS input",
        "scope_region": "neocortex layer II/III",
        "study_system": "mouse neocortex, Kv3.1/Kv3.2 double KO",
        "taxonomic_level": "cell type level",
        "scope_population": "FS cell synapses",
        "value_source_sentence": "in Kv3.1/Kv3.2 double knock-out mice, spike-evoked GABA release was larger, and the PPR was lower than in wild-type mice.",
        "experimental_conditions": "FS cell-to-pyramidal cell paired recordings"
      }
    ],
    "comparison_id": "kv3-knockout-phenotypes",
    "comparison_name": "Impact of Kv3 subunit knockouts on fast-spiking phenotype across studies",
    "comparison_type": "convergent evidence",
    "what_it_reveals": "Progressive severity of FS phenotype disruption with increasing Kv3 gene loss: Kv3.1 single KO shows moderate 21% AP broadening with preserved FS core, Kv3.2 KO shows layer-specific impairment matching expression pattern, and Kv3.1/Kv3.2 DKO shows enhanced GABA release consistent with broadened presynaptic APs. This demonstrates both redundancy and complementarity within the Kv3 family.",
    "homogeneity_check": {
      "caveats": [
        "Different brain regions (RTN vs neocortex)",
        "Different metrics (AP duration vs firing rate vs GABA release)",
        "Single vs double knockout comparisons",
        "Kv3.1 KO study in RTN, others in cortex"
      ],
      "n_definition_uniform": "false",
      "scope_region_uniform": "false",
      "taxonomic_level_uniform": "true",
      "scope_population_uniform": "true"
    },
    "suggested_plot_type": "grouped bar"
  },
  "section_id": "section_05_evidence_package",
  "source_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewPV/blob/df9fc7e8d455b084152c9d713558dae0013cef21/evidence/section_05_evidence_package.json",
  "target_ref": "wiki_page:computationalreviewpv-05",
  "review_repo": "ComputationalReviewPV",
  "section_ref": "wiki_page:computationalreviewpv-05",
  "source_path": "evidence/section_05_evidence_package.json",
  "source_refs": [
    "paper:paper-08e60421a6c7",
    "paper:paper-20a89635af94",
    "paper:paper-f2a5716f0156"
  ],
  "section_title": "Intrinsic Electrophysiology: The Fast-Spiking Phenotype and Its Variants",
  "source_policy": {
    "mode": "public_source_pointer_with_short_context",
    "notes": [
      "Local review repositories are read-only inputs.",
      "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
    ],
    "source_commit_sha": "df9fc7e8d455b084152c9d713558dae0013cef21",
    "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewPV"
  },
  "generation_status": "complete",
  "review_bundle_ref": "analysis_bundle:ab-e6261c8263e7",
  "origin_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewPV/blob/df9fc7e8d455b084152c9d713558dae0013cef21/evidence/section_05_evidence_package.json",
  "commit_sha": "df9fc7e8d455b084152c9d713558dae0013cef21",
  "created_by": "persona-jerome-lecoq-gbo-neuroscience",
  "repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewPV"
}