GAD67/PV mRNA reductions across cortical regions in schizophrenia

{
  "papers": [
    {
      "n": 15,
      "doi": "10.1523/jneurosci.23-15-06315.2003",
      "value": "55%",
      "method": "dual-label in situ hybridization",
      "metric": "Percentage of PV neurons with detectable GAD67 mRNA",
      "n_analyzed": 15,
      "ci_or_error": null,
      "text_access": "abstract_only",
      "n_definition": "subject pairs (schizophrenia vs matched control)",
      "scope_region": "prefrontal cortex area 9",
      "study_system": "human postmortem PFC area 9",
      "taxonomic_level": "single cell type (PV neurons)",
      "scope_population": "PV mRNA-positive neurons",
      "value_source_sentence": "In subjects with schizophrenia only 55% of PV mRNA-positive neurons had detectable levels of GAD67 mRNA.",
      "experimental_conditions": "matched pairs schizophrenia vs control"
    },
    {
      "n": 12,
      "doi": "10.1176/appi.ajp.2007.07081223",
      "value": "largest reductions among GABA transcripts examined",
      "method": "real-time quantitative PCR",
      "metric": "Relative reduction of PV mRNA across cortical areas",
      "n_analyzed": 12,
      "ci_or_error": null,
      "text_access": "fulltext",
      "n_definition": "subject pairs",
      "scope_region": "4 cortical areas (DLPFC, ACC, motor, visual)",
      "study_system": "human postmortem 4 cortical areas (DLPFC, ACC, M1, V1)",
      "taxonomic_level": "transcript level (PV, SST, GAD67, GAT-1, CR)",
      "scope_population": "all neurons in region",
      "value_source_sentence": "The largest reductions were detected for mRNA encoding somatostatin and parvalbumin, followed by moderate decreases in mRNA expression for the 67-kilodalton isoform of glutamic acid decarboxylase.",
      "experimental_conditions": "schizophrenia vs matched normal comparison subjects"
    },
    {
      "n": 0,
      "doi": "10.1016/j.tins.2011.10.004",
      "value": "~50%",
      "method": "review synthesis of in situ hybridization data",
      "metric": "Proportion of PV neurons with lower GAD67 mRNA",
      "n_analyzed": 0,
      "ci_or_error": null,
      "text_access": "fulltext",
      "n_definition": "review summarizing multiple cohorts",
      "scope_region": "DLPFC layer 3",
      "study_system": "human postmortem DLPFC",
      "taxonomic_level": "single cell type (PV neurons)",
      "scope_population": "PV-positive neurons",
      "value_source_sentence": "In the DLPFC, GAD67 mRNA levels are lower in schizophrenia, and this deficit appears to be present in ~50% of PV neurons.",
      "experimental_conditions": "schizophrenia vs control"
    }
  ],
  "comparison_id": "gad67-reduction-across-cortical-regions-schizophrenia",
  "comparison_name": "GAD67/PV mRNA reductions across cortical regions in schizophrenia",
  "comparison_type": "convergent evidence",
  "what_it_reveals": "GAD67 reduction in PV neurons is remarkably consistent (~50% of PV cells affected) across studies and cortical regions, supporting a core pathological feature of schizophrenia",
  "homogeneity_check": {
    "caveats": [
      "One paper examines 4 cortical areas while others focus on DLPFC only",
      "One entry is from a review synthesis rather than original data",
      "n_definition varies: some count subject pairs, one is a review"
    ],
    "n_definition_uniform": "false",
    "scope_region_uniform": "false",
    "taxonomic_level_uniform": "true",
    "scope_population_uniform": "true"
  },
  "suggested_plot_type": "grouped bar"
}

[
  "paper:paper-6d36973b50ab",
  "paper:paper-9febfa10db1a",
  "paper:paper-fe0468c6576e"
]

{
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  "notes": [
    "Local review repositories are read-only inputs.",
    "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
  ],
  "source_commit_sha": "df9fc7e8d455b084152c9d713558dae0013cef21",
  "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewPV"
}