Input Resistance of SST Interneuron Subtypes Across Studies

Reveals the heterogeneity of passive membrane properties within the SST population and whether different subtypes (Martinotti, non-Martinotti, layer-specific) have systematically different input resistances, which impacts their sensitivity to synaptic input.

{
  "papers": [
    {
      "doi": "10.1523/eneuro.0204-23.2023",
      "value": "resistance: 5–8 MΩ",
      "method": "whole-cell patch-clamp",
      "metric": "input resistance",
      "cite_key": "Hostetler2023b",
      "condition": "in vitro",
      "study_system": "mouse, cortex, in vivo",
      "value_source_sentence": "For whole-cell recordings, glass micropipettes (typical resistance, 5–8 MΩ) were filled with an intracellular solution containing the following (in mm): K-gluconate 134, KCl 3.5, CaCl2 0.1, HEPES 10, EGTA 1.1, Mg-ATP 4, phosphocreatine-Tris 10, and 2 mg/ml biocytin, adjusted to pH 7.25 and 290 mOsm."
    },
    {
      "doi": "10.1113/jp286439",
      "value": "resistance: 4–7 MΩ",
      "method": "whole-cell patch-clamp",
      "metric": "input resistance",
      "cite_key": "Bogaj2025",
      "condition": "in vitro",
      "study_system": "mouse, cortex, in vitro",
      "value_source_sentence": "Recordings were obtained via borosilicate glass electrodes (4–7 MΩ resistance) when filled with an internal solution consisting of (mM): 125 potassium gluconate, 2 KCl, 10 Hepes, 0.5 EGTA, 4 MgATP and 0.3 NaGTP, at pH 7.2, 270 mOsm (Urban‐Ciecko et al., 2015, 2010, 2018)."
    },
    {
      "doi": "10.1093/cercor/bhaa201",
      "value": "resistance: 7 MΩ",
      "method": "whole-cell patch-clamp",
      "metric": "input resistance",
      "cite_key": "Ding2021",
      "condition": "in vitro",
      "study_system": "rat, cortex",
      "value_source_sentence": "The recordings were performed using patch pipettes of resistance between 5 and 7 MΩ."
    },
    {
      "doi": "10.1038/s41598-024-70278-w",
      "value": "resistance: 3–5 MΩ",
      "method": "whole-cell patch-clamp",
      "metric": "input resistance",
      "cite_key": "Wiera2024",
      "condition": "in vitro",
      "study_system": "rat, hippocampus",
      "value_source_sentence": "Patch pipettes (borosilicate glass, OD 1.5 mm, ID 0.86 mm, tip resistance 3–5 MΩ) were pulled using a Sutter P-97 micropipette puller."
    },
    {
      "doi": "10.1038/s41380-024-02517-5",
      "value": "resistance: 3–4 MΩ",
      "method": "whole-cell patch-clamp",
      "metric": "input resistance",
      "cite_key": "Campbell2024",
      "condition": "in vitro",
      "study_system": "mouse, hippocampus",
      "value_source_sentence": "Whole-cell recordings were obtained from pyramidal neurons in CA1 at RT using borosilicate patch pipettes (Harvard apparatus, 30–0068) pulled to a resistance of 3–4 MΩ filled with internal solution containing (in mM): 130 CsCl, 4 NaCl, 2 MgCl2, 1.1 EGTA, 5 HEPES, 2 Na2ATP, 5 sodium creatine phosphat"
    },
    {
      "doi": "10.3389/fphar.2025.1640921",
      "value": "resistance: 4–6 MΩ",
      "method": "whole-cell patch-clamp",
      "metric": "input resistance",
      "cite_key": "Wilson2026",
      "condition": "in vitro",
      "study_system": "mouse, cortex, in vitro",
      "value_source_sentence": "Pipettes were filled with K-gluconate based solution (in mM: 142 K-gluconate, 4 KCl, 0.5 EGTA, 10 HEPES, 2 MgCl2, 2 Na2ATP, 0.3 Na2GTP, 10 Na2Phosphocreatine, 2.7 Biocytin, pH = 7.4, 290–310 mOsm), giving a resistance of 4–6 MΩ."
    }
  ],
  "comparison_id": "sst-input-resistance-across-studies",
  "comparison_name": "Input Resistance of SST Interneuron Subtypes Across Studies",
  "comparison_type": "cross-study conflict",
  "what_it_reveals": "Reveals the heterogeneity of passive membrane properties within the SST population and whether different subtypes (Martinotti, non-Martinotti, layer-specific) have systematically different input resistances, which impacts their sensitivity to synaptic input.",
  "homogeneity_check": {
    "caveats": [
      "Different recording temperatures (room temp vs 32-34°C)",
      "Different internal solutions across labs",
      "Different definitions of SST subtypes",
      "Age of animals varies"
    ],
    "comparable": false
  },
  "suggested_plot_type": "forest plot"
}

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  "paper:paper-3e673e05c943",
  "paper:paper-471271479547",
  "paper:paper-60735da5f5a2",
  "paper:paper-6634769ed2ab",
  "paper:paper-7d76df87a749"
]

{
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  "notes": [
    "Local review repositories are read-only inputs.",
    "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
  ],
  "source_commit_sha": "89b7e9787cd90e942b0adb531d549af3ddad30f1",
  "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewSST"
}