Version history

{
  "kind": "infographic",
  "prompt": "Reveals the heterogeneity of passive membrane properties within the SST population and whether different subtypes (Martinotti, non-Martinotti, layer-specific) have systematically different input resistances, which impacts their sensitivity to synaptic input.",
  "provider": "other",
  "raw_fields": {
    "papers": [
      {
        "doi": "10.1523/eneuro.0204-23.2023",
        "value": "resistance: 5–8 MΩ",
        "method": "whole-cell patch-clamp",
        "metric": "input resistance",
        "cite_key": "Hostetler2023b",
        "condition": "in vitro",
        "study_system": "mouse, cortex, in vivo",
        "value_source_sentence": "For whole-cell recordings, glass micropipettes (typical resistance, 5–8 MΩ) were filled with an intracellular solution containing the following (in mm): K-gluconate 134, KCl 3.5, CaCl2 0.1, HEPES 10, EGTA 1.1, Mg-ATP 4, phosphocreatine-Tris 10, and 2 mg/ml biocytin, adjusted to pH 7.25 and 290 mOsm."
      },
      {
        "doi": "10.1113/jp286439",
        "value": "resistance: 4–7 MΩ",
        "method": "whole-cell patch-clamp",
        "metric": "input resistance",
        "cite_key": "Bogaj2025",
        "condition": "in vitro",
        "study_system": "mouse, cortex, in vitro",
        "value_source_sentence": "Recordings were obtained via borosilicate glass electrodes (4–7 MΩ resistance) when filled with an internal solution consisting of (mM): 125 potassium gluconate, 2 KCl, 10 Hepes, 0.5 EGTA, 4 MgATP and 0.3 NaGTP, at pH 7.2, 270 mOsm (Urban‐Ciecko et al., 2015, 2010, 2018)."
      },
      {
        "doi": "10.1093/cercor/bhaa201",
        "value": "resistance: 7 MΩ",
        "method": "whole-cell patch-clamp",
        "metric": "input resistance",
        "cite_key": "Ding2021",
        "condition": "in vitro",
        "study_system": "rat, cortex",
        "value_source_sentence": "The recordings were performed using patch pipettes of resistance between 5 and 7 MΩ."
      },
      {
        "doi": "10.1038/s41598-024-70278-w",
        "value": "resistance: 3–5 MΩ",
        "method": "whole-cell patch-clamp",
        "metric": "input resistance",
        "cite_key": "Wiera2024",
        "condition": "in vitro",
        "study_system": "rat, hippocampus",
        "value_source_sentence": "Patch pipettes (borosilicate glass, OD 1.5 mm, ID 0.86 mm, tip resistance 3–5 MΩ) were pulled using a Sutter P-97 micropipette puller."
      },
      {
        "doi": "10.1038/s41380-024-02517-5",
        "value": "resistance: 3–4 MΩ",
        "method": "whole-cell patch-clamp",
        "metric": "input resistance",
        "cite_key": "Campbell2024",
        "condition": "in vitro",
        "study_system": "mouse, hippocampus",
        "value_source_sentence": "Whole-cell recordings were obtained from pyramidal neurons in CA1 at RT using borosilicate patch pipettes (Harvard apparatus, 30–0068) pulled to a resistance of 3–4 MΩ filled with internal solution containing (in mM): 130 CsCl, 4 NaCl, 2 MgCl2, 1.1 EGTA, 5 HEPES, 2 Na2ATP, 5 sodium creatine phosphat"
      },
      {
        "doi": "10.3389/fphar.2025.1640921",
        "value": "resistance: 4–6 MΩ",
        "method": "whole-cell patch-clamp",
        "metric": "input resistance",
        "cite_key": "Wilson2026",
        "condition": "in vitro",
        "study_system": "mouse, cortex, in vitro",
        "value_source_sentence": "Pipettes were filled with K-gluconate based solution (in mM: 142 K-gluconate, 4 KCl, 0.5 EGTA, 10 HEPES, 2 MgCl2, 2 Na2ATP, 0.3 Na2GTP, 10 Na2Phosphocreatine, 2.7 Biocytin, pH = 7.4, 290–310 mOsm), giving a resistance of 4–6 MΩ."
      }
    ],
    "comparison_id": "sst-input-resistance-across-studies",
    "comparison_name": "Input Resistance of SST Interneuron Subtypes Across Studies",
    "comparison_type": "cross-study conflict",
    "what_it_reveals": "Reveals the heterogeneity of passive membrane properties within the SST population and whether different subtypes (Martinotti, non-Martinotti, layer-specific) have systematically different input resistances, which impacts their sensitivity to synaptic input.",
    "homogeneity_check": {
      "caveats": [
        "Different recording temperatures (room temp vs 32-34°C)",
        "Different internal solutions across labs",
        "Different definitions of SST subtypes",
        "Age of animals varies"
      ],
      "comparable": false
    },
    "suggested_plot_type": "forest plot"
  },
  "section_id": "section_05_evidence_package",
  "source_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewSST/blob/89b7e9787cd90e942b0adb531d549af3ddad30f1/evidence/section_05_evidence_package.json",
  "target_ref": "wiki_page:computationalreviewsst-05",
  "review_repo": "ComputationalReviewSST",
  "section_ref": "wiki_page:computationalreviewsst-05",
  "source_path": "evidence/section_05_evidence_package.json",
  "source_refs": [
    "paper:paper-3ca05a41fd8f",
    "paper:paper-3e673e05c943",
    "paper:paper-471271479547",
    "paper:paper-60735da5f5a2",
    "paper:paper-6634769ed2ab",
    "paper:paper-7d76df87a749"
  ],
  "section_title": "Intrinsic Electrophysiology",
  "source_policy": {
    "mode": "public_source_pointer_with_short_context",
    "notes": [
      "Local review repositories are read-only inputs.",
      "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
    ],
    "source_commit_sha": "89b7e9787cd90e942b0adb531d549af3ddad30f1",
    "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewSST"
  },
  "generation_status": "complete",
  "review_bundle_ref": "analysis_bundle:ab-8466d095488a",
  "origin_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewSST/blob/89b7e9787cd90e942b0adb531d549af3ddad30f1/evidence/section_05_evidence_package.json",
  "commit_sha": "89b7e9787cd90e942b0adb531d549af3ddad30f1",
  "created_by": "persona-jerome-lecoq-gbo-neuroscience",
  "repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewSST"
}