Version history

{
  "kind": "infographic",
  "prompt": "Quantifies the present envelope (yield, depth, frame rate) of 2P/widefield voltage imaging in mouse cortex relevant to E→E claims.",
  "provider": "other",
  "raw_fields": {
    "papers": [
      {
        "n": 0,
        "doi": "10.1117/1.nph.11.3.035007",
        "value": "~12",
        "method": "2P voltage imaging modeling",
        "metric": "max simultaneous neurons (2P, SNR=10, >300 µm)",
        "n_analyzed": "",
        "ci_or_error": "",
        "text_access": "abstract_only",
        "n_definition": "upper-bound count from biophysical model",
        "scope_region": "mouse cortex",
        "study_system": "Mouse cortex (model)",
        "taxonomic_level": "broad category",
        "scope_population": "voltage indicator cells",
        "value_source_sentence": "2P voltage imaging using an 80-MHz source can record from no more than ∼ 12  neurons simultaneously.",
        "experimental_conditions": "JEDI-2P, SNR 10, 1 kHz, 200 mW, >300 µm depth"
      },
      {
        "n": 0,
        "doi": "10.1016/j.cell.2022.07.013",
        "value": ">400 µm",
        "method": "2P voltage imaging (ULoVE)",
        "metric": "spike detection depth (ULoVE)",
        "n_analyzed": "",
        "ci_or_error": "",
        "text_access": "abstract_only",
        "n_definition": "max imaging depth with single-spike detection",
        "scope_region": "mouse cortex",
        "study_system": "Awake mouse cortex",
        "taxonomic_level": "broad category",
        "scope_population": "individual cortical neurons",
        "value_source_sentence": "Finally, ULoVE recording of JEDI-2P can robustly detect spikes at depths exceeding 400 μm and report voltage correlations in pairs of neurons.",
        "experimental_conditions": "JEDI-2P, ULoVE random-access 2P"
      },
      {
        "n": 0,
        "doi": "10.1364/boe.576516",
        "value": "530 Hz",
        "method": "Miniaturized widefield voltage imaging",
        "metric": "miniaturized widefield frame rate",
        "n_analyzed": "",
        "ci_or_error": "SNR >3",
        "text_access": "abstract_only",
        "n_definition": "frame rate of imaging system",
        "scope_region": "mouse cortex",
        "study_system": "In vivo mouse",
        "taxonomic_level": "broad category",
        "scope_population": "Voltron2-expressing cells",
        "value_source_sentence": "We show it is capable of imaging <i>in vivo</i> voltage spikes from Voltron2 with a spike peak-to-noise ratio >3 at a framerate of 530 Hz.",
        "experimental_conditions": "Voltron2, 250 µm FOV, 16.4 g headpiece"
      },
      {
        "n": 0,
        "doi": "10.1016/j.crmeth.2025.101221",
        "value": "400 Hz",
        "method": "Miniaturized 2P voltage imaging",
        "metric": "voltage frame rate (freely moving)",
        "n_analyzed": "",
        "ci_or_error": "",
        "text_access": "abstract_only",
        "n_definition": "voltage-imaging frame rate",
        "scope_region": "mouse cortex",
        "study_system": "Freely moving mouse cortex",
        "taxonomic_level": "broad category",
        "scope_population": "GEVI-expressing cells",
        "value_source_sentence": "The increased speed of M-MINI2Ps also enables two-photon voltage imaging at 400 Hz over a 380 × 150 μm<sup>2</sup> FOV in freely moving mice.",
        "experimental_conditions": "M-MINI2P miniature 2P, 380×150 µm² FOV"
      }
    ],
    "audit_issues": [
      {
        "dimension": "metric_definition",
        "description": "Rows report different quantities on the y-axis (max simultaneous neurons, max imaging depth, frame rate). A 'grouped bar' over heterogeneous quantities is not a valid comparison.",
        "entries_affected": [
          "10.1117/1.nph.11.3.035007",
          "10.1016/j.cell.2022.07.013",
          "10.1364/boe.576516",
          "10.1016/j.crmeth.2025.101221"
        ]
      },
      {
        "dimension": "study_system",
        "description": "One row is a biophysical model upper bound (10.1117/1.nph.11.3.035007); others are empirical 2P/widefield voltage imaging.",
        "entries_affected": [
          "10.1117/1.nph.11.3.035007"
        ]
      }
    ],
    "audit_verdict": "SPLIT",
    "comparison_id": "2p-voltage-imaging-limits",
    "comparison_name": "Present-day depth, neuron-count, and frame-rate of in-vivo 2P voltage imaging in mouse cortex",
    "comparison_type": "convergent evidence",
    "what_it_reveals": "Quantifies the present envelope (yield, depth, frame rate) of 2P/widefield voltage imaging in mouse cortex relevant to E→E claims.",
    "homogeneity_check": {
      "caveats": [
        "Metrics are heterogeneous (neuron count vs depth vs frame rate); pooled only as a depiction of the resolution envelope, not a single comparable quantity.",
        "One row is a biophysical-model upper bound rather than empirical measurement (10.1117/1.nph.11.3.035007)."
      ],
      "n_definition_uniform": "false",
      "scope_region_uniform": "true",
      "taxonomic_level_uniform": "true",
      "scope_population_uniform": "false"
    },
    "suggested_plot_type": "grouped bar",
    "mandatory_caption_caveats": [
      "Rows do not share a metric (neuron count vs. depth vs. frame rate); the figure should be read as a multi-axis envelope, not a single comparable quantity.",
      "One row is a biophysical model upper bound; others are empirical measurements.",
      "Phase 7 writer: SPLIT verdict — implement as: Split into separate panels by quantity (neuron count, depth, frame rate) or convert to a table where each row reports all three quantities. Clearly distinguish model upper bounds from empirical measurements."
    ]
  },
  "section_id": "section_15",
  "source_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence/blob/79ce062d54a924ce05953ec90aa9d26044d2b48f/evidence/section_15_evidence_package.json",
  "target_ref": "wiki_page:computationalreviewrecurrence-15-methods-limits",
  "review_repo": "ComputationalReviewRecurrence",
  "section_ref": "wiki_page:computationalreviewrecurrence-15-methods-limits",
  "source_path": "evidence/section_15_evidence_package.json",
  "source_refs": [
    "paper:paper-07e278a78498",
    "paper:paper-2daa8d033efa",
    "paper:paper-d123a7539f4f",
    "paper:paper-ed927db80f97"
  ],
  "section_title": "15. Methodological limits and emerging tools — what current mouse-cortex tools cannot yet measure about E→E recurrence (subthreshold network activity, fast plasticity in vivo, millimetre-scale dynamic connectomes), and what is on the near horizon",
  "source_policy": {
    "mode": "public_source_pointer_with_short_context",
    "notes": [
      "Local review repositories are read-only inputs.",
      "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
    ],
    "source_commit_sha": "79ce062d54a924ce05953ec90aa9d26044d2b48f",
    "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence"
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  "generation_status": "complete",
  "review_bundle_ref": "analysis_bundle:ab-d9c479db9be9",
  "origin_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence/blob/79ce062d54a924ce05953ec90aa9d26044d2b48f/evidence/section_15_evidence_package.json",
  "commit_sha": "79ce062d54a924ce05953ec90aa9d26044d2b48f",
  "created_by": "persona-jerome-lecoq-gbo-neuroscience",
  "repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence"
}