{
"papers": [
{
"n": 0,
"doi": "10.1016/j.neuron.2017.06.029",
"value": "higher in hippocampus than striatum; P = 0.0469 | striatum > hippocampus; P = 0.0199",
"method": "two-photon GCaMP imaging in acute slice | acute slice GCaMP + Ca2+-free manipulations",
"metric": "spontaneous Ca2+ signal frequency (hippocampus vs striatum) | basal Ca2+ dependence on extracellular entry (striatum vs hippocampus)",
"text_access": "fulltext",
"n_definition": "ROIs from dl-striatal and hippocampal CA1 s.r. astrocytes | astrocyte ROIs per slice",
"scope_region": "dl-striatum vs hippocampus CA1",
"study_system": "mouse acute slice, dorsolateral striatum vs hippocampal CA1 stratum radiatum astrocytes, GCaMP6 imaging | mouse acute slice, striatum vs hippocampus CA1, GCaMP imaging",
"taxonomic_level": "regional",
"scope_population": "all astrocytes imaged",
"value_source_sentence": "The example traces and pooled data show dramatic reductions in the frequency of spontaneous Ca 2+ signals in all compartments for both d.l. striatal and hippocampal CA1 s.r. astrocytes ( 2+ free buffers was equivalent between the striatum and hippocampus, indicating similar dependence on Ca 2+ entry for spontaneous signals ( Spontaneous Ca 2+ signal frequency was higher in the hippocampus than striatum under control conditions ( P = 0.0469; P = 0.0165), and in Ca 2+ -free ( P = 0.0228; P = 0.0052). | Astrocytes from the striatum relied more heavily on extracellular entry for basal Ca 2+ levels than astrocytes from the hippocampus ( P = 0.0199).",
"experimental_conditions": "control TTX buffers; spontaneous microdomain Ca2+ | store-depletion protocols; Ca2+-free buffers"
},
{
"n": 0,
"doi": "10.1016/j.neuron.2018.08.015",
"value": ">80%",
"method": "AAV astrocyte-specific Ca2+ attenuator; GCaMP imaging",
"metric": "striatal astrocyte Ca2+ attenuation by hPMCA2w/b expression",
"n_analyzed": null,
"ci_or_error": null,
"text_access": "fulltext",
"n_definition": "astrocytes per slice",
"scope_region": "dorsolateral striatum",
"study_system": "mouse dorsolateral striatum astrocytes",
"taxonomic_level": "regional",
"scope_population": "astrocytes transduced with hPMCA2w/b (~90% of DLS astrocytes)",
"value_source_sentence": "For PE-evoked responses, both hPMCA2w/b and IP3R2 deletion similarly (>80%) attenuated the responses, but the degree of reduction was statistically greater in IP3R2 deletion mice ( Reducing striatal astrocyte calcium-dependent signaling leads to excessive self-grooming We microinjected hPMCA2w/b (or control AAVs) bilaterally into the dorsolateral striatum to determine how attenuation of Ca 2+ -dependent signaling affected behavior.",
"experimental_conditions": "phenylephrine (PE)-evoked Ca2+; AAV-hPMCA2w/b"
},
{
"n": 0,
"doi": "10.1016/j.neuron.2016.12.034",
"value": "24% increase",
"method": "GCaMP Lck imaging of astrocyte processes",
"metric": "NE-evoked increase in active microdomains (cortex)",
"n_analyzed": null,
"ci_or_error": null,
"text_access": "fulltext",
"n_definition": "microdomains per field of view",
"scope_region": "visual cortex",
"study_system": "mouse visual cortex astrocytes",
"taxonomic_level": "regional",
"scope_population": "cortical astrocytes, IP3R2-independent microdomains",
"value_source_sentence": "Unexpectedly, NE (10 μM) elicited a small increase (24%) in the number of active microdomains ( 2+ transients ( To determine if IP 3 R2-independent microdomain Ca 2+ transients also occur in astrocytes in vivo , we monitored spontaneous and NE-induced Ca 2+ transients in control ( GLAST-mGC3 ) and IP3R2−/− mice ( GLAST-mGC3;IP3R2−/− ) in primary visual cortex using two photon imaging through a cranial window without anesthesia ( 2+ transients that were unsynchronized ( GLAST-mGC3;IP3R2−/− mice in vivo was also attenuated relative to controls ( 2+ during locomotion, as NE is released when the state of arousal of the mice is increased ( in vivo NE release led to a 4.5x increase in number of microdomain events ( 2+ transients (z-score) ( IP3R2−/− mice, enforced locomotion increased the frequency of microdomain events by 1.9x, but did not alter their amplitude ( in vivo phenomenon that persists without IP 3 R2-mediated Ca 2+ release.",
"experimental_conditions": "acute slice, norepinephrine 10 μM"
},
{
"n": 4,
"doi": "10.1016/j.neuron.2014.04.038",
"value": "R = 0.0352, p = 0.4365 (495 events from 4 mice) — not correlated with speed",
"method": "in vivo two-photon GCaMP3",
"metric": "Bergmann glia Ca2+ locomotion correlation",
"n_analyzed": "495 events",
"ci_or_error": "R = 0.0352",
"text_access": "fulltext",
"n_definition": "mice / events analyzed",
"scope_region": "cerebellum (Bergmann glia)",
"study_system": "mouse cerebellum, Bergmann glia, in vivo GCaMP3",
"taxonomic_level": "regional (cerebellar glia subtype)",
"scope_population": "Bergmann glia expressing R26-lsl-GCaMP3",
"value_source_sentence": "In accordance with previous findings ( 2+ in Bergmann glia that persisted for many seconds after cessation of movement ( 2+ response was not correlated with locomotion speed (R = 0.0352, p = 0.4365, 495 events from 4 mice), and locomotion often did not trigger activation of Bergmann glia (212 failures in 707 locomotion events).",
"experimental_conditions": "treadmill locomotion"
}
],
"comparison_id": "regional-spontaneous-ca-frequency",
"comparison_name": "Astrocyte spontaneous Ca2+ signal frequency and source across brain regions",
"comparison_type": "convergent evidence",
"what_it_reveals": "Functional Ca2+ dynamics in astrocytes are region-specific: hippocampal astrocytes have higher spontaneous frequency than striatum, striatal astrocytes rely more on extracellular Ca2+ entry, Bergmann glia respond to behavioral state without speed-proportionality, and cortical astrocyte microdomains respond to NE.",
"homogeneity_check": {
"caveats": [
"Metrics differ (frequency, dependence, attenuation %, correlation) across entries.",
"Methods differ: acute slice (Chai, Yu, Srinivasan) vs in vivo (Nimmerjahn).",
"GECIs differ (GCaMP3 vs GCaMP6s vs Lck-GCaMP).",
"Striatum entries are dorsolateral; hippocampus is CA1 s.r.; cerebellum is Bergmann glia — compartment types are not identical."
],
"n_definition_uniform": "false",
"scope_region_uniform": "false",
"taxonomic_level_uniform": "false",
"scope_population_uniform": "false"
},
"suggested_plot_type": "grouped bar"
}