Version history

{
  "kind": "infographic",
  "prompt": "Functional Ca2+ dynamics in astrocytes are region-specific: hippocampal astrocytes have higher spontaneous frequency than striatum, striatal astrocytes rely more on extracellular Ca2+ entry, Bergmann glia respond to behavioral state without speed-proportionality, and cortical astrocyte microdomains respond to NE.",
  "provider": "other",
  "raw_fields": {
    "papers": [
      {
        "n": 0,
        "doi": "10.1016/j.neuron.2017.06.029",
        "value": "higher in hippocampus than striatum; P = 0.0469 | striatum > hippocampus; P = 0.0199",
        "method": "two-photon GCaMP imaging in acute slice | acute slice GCaMP + Ca2+-free manipulations",
        "metric": "spontaneous Ca2+ signal frequency (hippocampus vs striatum) | basal Ca2+ dependence on extracellular entry (striatum vs hippocampus)",
        "text_access": "fulltext",
        "n_definition": "ROIs from dl-striatal and hippocampal CA1 s.r. astrocytes | astrocyte ROIs per slice",
        "scope_region": "dl-striatum vs hippocampus CA1",
        "study_system": "mouse acute slice, dorsolateral striatum vs hippocampal CA1 stratum radiatum astrocytes, GCaMP6 imaging | mouse acute slice, striatum vs hippocampus CA1, GCaMP imaging",
        "taxonomic_level": "regional",
        "scope_population": "all astrocytes imaged",
        "value_source_sentence": "The example traces and pooled data show dramatic reductions in the frequency of spontaneous Ca 2+ signals in all compartments for both d.l. striatal and hippocampal CA1 s.r. astrocytes ( 2+ free buffers was equivalent between the striatum and hippocampus, indicating similar dependence on Ca 2+ entry for spontaneous signals ( Spontaneous Ca 2+ signal frequency was higher in the hippocampus than striatum under control conditions ( P = 0.0469; P = 0.0165), and in Ca 2+ -free ( P = 0.0228; P = 0.0052). | Astrocytes from the striatum relied more heavily on extracellular entry for basal Ca 2+ levels than astrocytes from the hippocampus ( P = 0.0199).",
        "experimental_conditions": "control TTX buffers; spontaneous microdomain Ca2+ | store-depletion protocols; Ca2+-free buffers"
      },
      {
        "n": 0,
        "doi": "10.1016/j.neuron.2018.08.015",
        "value": ">80%",
        "method": "AAV astrocyte-specific Ca2+ attenuator; GCaMP imaging",
        "metric": "striatal astrocyte Ca2+ attenuation by hPMCA2w/b expression",
        "n_analyzed": null,
        "ci_or_error": null,
        "text_access": "fulltext",
        "n_definition": "astrocytes per slice",
        "scope_region": "dorsolateral striatum",
        "study_system": "mouse dorsolateral striatum astrocytes",
        "taxonomic_level": "regional",
        "scope_population": "astrocytes transduced with hPMCA2w/b (~90% of DLS astrocytes)",
        "value_source_sentence": "For PE-evoked responses, both hPMCA2w/b and IP3R2 deletion similarly (>80%) attenuated the responses, but the degree of reduction was statistically greater in IP3R2 deletion mice ( Reducing striatal astrocyte calcium-dependent signaling leads to excessive self-grooming We microinjected hPMCA2w/b (or control AAVs) bilaterally into the dorsolateral striatum to determine how attenuation of Ca 2+ -dependent signaling affected behavior.",
        "experimental_conditions": "phenylephrine (PE)-evoked Ca2+; AAV-hPMCA2w/b"
      },
      {
        "n": 0,
        "doi": "10.1016/j.neuron.2016.12.034",
        "value": "24% increase",
        "method": "GCaMP Lck imaging of astrocyte processes",
        "metric": "NE-evoked increase in active microdomains (cortex)",
        "n_analyzed": null,
        "ci_or_error": null,
        "text_access": "fulltext",
        "n_definition": "microdomains per field of view",
        "scope_region": "visual cortex",
        "study_system": "mouse visual cortex astrocytes",
        "taxonomic_level": "regional",
        "scope_population": "cortical astrocytes, IP3R2-independent microdomains",
        "value_source_sentence": "Unexpectedly, NE (10 μM) elicited a small increase (24%) in the number of active microdomains ( 2+ transients ( To determine if IP 3 R2-independent microdomain Ca 2+ transients also occur in astrocytes in vivo , we monitored spontaneous and NE-induced Ca 2+ transients in control ( GLAST-mGC3 ) and IP3R2−/− mice ( GLAST-mGC3;IP3R2−/− ) in primary visual cortex using two photon imaging through a cranial window without anesthesia ( 2+ transients that were unsynchronized ( GLAST-mGC3;IP3R2−/− mice in vivo was also attenuated relative to controls ( 2+ during locomotion, as NE is released when the state of arousal of the mice is increased ( in vivo NE release led to a 4.5x increase in number of microdomain events ( 2+ transients (z-score) ( IP3R2−/− mice, enforced locomotion increased the frequency of microdomain events by 1.9x, but did not alter their amplitude ( in vivo phenomenon that persists without IP 3 R2-mediated Ca 2+ release.",
        "experimental_conditions": "acute slice, norepinephrine 10 μM"
      },
      {
        "n": 4,
        "doi": "10.1016/j.neuron.2014.04.038",
        "value": "R = 0.0352, p = 0.4365 (495 events from 4 mice) — not correlated with speed",
        "method": "in vivo two-photon GCaMP3",
        "metric": "Bergmann glia Ca2+ locomotion correlation",
        "n_analyzed": "495 events",
        "ci_or_error": "R = 0.0352",
        "text_access": "fulltext",
        "n_definition": "mice / events analyzed",
        "scope_region": "cerebellum (Bergmann glia)",
        "study_system": "mouse cerebellum, Bergmann glia, in vivo GCaMP3",
        "taxonomic_level": "regional (cerebellar glia subtype)",
        "scope_population": "Bergmann glia expressing R26-lsl-GCaMP3",
        "value_source_sentence": "In accordance with previous findings ( 2+ in Bergmann glia that persisted for many seconds after cessation of movement ( 2+ response was not correlated with locomotion speed (R = 0.0352, p = 0.4365, 495 events from 4 mice), and locomotion often did not trigger activation of Bergmann glia (212 failures in 707 locomotion events).",
        "experimental_conditions": "treadmill locomotion"
      }
    ],
    "comparison_id": "regional-spontaneous-ca-frequency",
    "comparison_name": "Astrocyte spontaneous Ca2+ signal frequency and source across brain regions",
    "comparison_type": "convergent evidence",
    "what_it_reveals": "Functional Ca2+ dynamics in astrocytes are region-specific: hippocampal astrocytes have higher spontaneous frequency than striatum, striatal astrocytes rely more on extracellular Ca2+ entry, Bergmann glia respond to behavioral state without speed-proportionality, and cortical astrocyte microdomains respond to NE.",
    "homogeneity_check": {
      "caveats": [
        "Metrics differ (frequency, dependence, attenuation %, correlation) across entries.",
        "Methods differ: acute slice (Chai, Yu, Srinivasan) vs in vivo (Nimmerjahn).",
        "GECIs differ (GCaMP3 vs GCaMP6s vs Lck-GCaMP).",
        "Striatum entries are dorsolateral; hippocampus is CA1 s.r.; cerebellum is Bergmann glia — compartment types are not identical."
      ],
      "n_definition_uniform": "false",
      "scope_region_uniform": "false",
      "taxonomic_level_uniform": "false",
      "scope_population_uniform": "false"
    },
    "suggested_plot_type": "grouped bar"
  },
  "section_id": "section_11_evidence_package",
  "source_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewAstrocytes/blob/1a55da0634a3bc04e5688792ed12141ce271d28e/evidence/section_11_evidence_package.json",
  "target_ref": "wiki_page:computationalreviewastrocytes-11",
  "review_repo": "ComputationalReviewAstrocytes",
  "section_ref": "wiki_page:computationalreviewastrocytes-11",
  "source_path": "evidence/section_11_evidence_package.json",
  "source_refs": [
    "paper:paper-23d7b31898ec",
    "paper:paper-54c454be2778",
    "paper:paper-9797394cf360",
    "paper:paper-c860b586a738"
  ],
  "section_title": "Regional Diversity of Astrocytes",
  "source_policy": {
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    "notes": [
      "Local review repositories are read-only inputs.",
      "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
    ],
    "source_commit_sha": "1a55da0634a3bc04e5688792ed12141ce271d28e",
    "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewAstrocytes"
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  "generation_status": "complete",
  "review_bundle_ref": "analysis_bundle:ab-029ee9411fe2",
  "origin_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewAstrocytes/blob/1a55da0634a3bc04e5688792ed12141ce271d28e/evidence/section_11_evidence_package.json",
  "commit_sha": "1a55da0634a3bc04e5688792ed12141ce271d28e",
  "created_by": "persona-jerome-lecoq-gbo-neuroscience",
  "repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewAstrocytes"
}