Zhang et al. (2021) used MERFISH to generate a molecularly defined and spatiall…

Details

scope: mouse MOp; MERFISH spatial transcriptomics
claim_text: Zhang et al. (2021) used MERFISH to generate a molecularly defined and spatially resolved cell atlas of mouse primary motor cortex (MOp), targeting 258 genes including canonical interneuron markers (Vip, Sst, Pvalb, Lamp5).
section_id: section_02
source_url: https://github.com/AllenNeuralDynamics/ComputationalReviewVIP/blob/95e761177f7d2ec565983d3307c14ec238f9677c/evidence/section_02_evidence_package.json
review_repo: ComputationalReviewVIP
section_ref: wiki_page:computationalreviewvip-02-classification
source_kind: review_finding
source_path: evidence/section_02_evidence_package.json
source_span: Here we used a single-cell transcriptome-imaging method, multiplexed error-robust fluorescence in situ hybridization (MERFISH), to generate a molecularly defined and spatially resolved cell atlas of the mouse primary motor cortex.
study_system: mouse MOp; MERFISH spatial transcriptomics
section_title: Molecular Identity and Transcriptomic Taxonomy
evidence_summary: MERFISH on 258-gene panel; identified Vip, Sncg, Lamp5 CGE subclasses preferentially in upper cortical layers, Sst/Pvalb in deeper; cluster correspondence with scRNA-seq/snRNA-seq.
review_bundle_ref: analysis_bundle:ab-2ce40c33e827
replication_status: replicated
review_package_ref: analysis_bundle:ab-2ce40c33e827
source_artifact_ref: wiki_page:computationalreviewvip-02-classification
origin_url: https://github.com/AllenNeuralDynamics/ComputationalReviewVIP/blob/95e761177f7d2ec565983d3307c14ec238f9677c/evidence/section_02_evidence_package.json
commit_sha: 95e761177f7d2ec565983d3307c14ec238f9677c
created_by: persona-jerome-lecoq-gbo-neuroscience
repository_url: https://github.com/AllenNeuralDynamics/ComputationalReviewVIP

Raw fields (4)

raw_fields

{
  "n": null,
  "doi": "10.1038/s41586-021-03705-x",
  "claim": "Zhang et al. (2021) used MERFISH to generate a molecularly defined and spatially resolved cell atlas of mouse primary motor cortex (MOp), targeting 258 genes including canonical interneuron markers (Vip, Sst, Pvalb, Lamp5).",
  "title": null,
  "cite_key": "Zhang2021",
  "evidence": "MERFISH on 258-gene panel; identified Vip, Sncg, Lamp5 CGE subclasses preferentially in upper cortical layers, Sst/Pvalb in deeper; cluster correspondence with scRNA-seq/snRNA-seq.",
  "effect_size": null,
  "text_access": "fulltext",
  "study_system": "mouse MOp; MERFISH spatial transcriptomics",
  "target_section": "section_02",
  "_source_cluster": "cluster_01_molecular_taxonomy",
  "replication_status": "replicated",
  "_source_cluster_index": 13,
  "claim_source_sentence": "Here we used a single-cell transcriptome-imaging method, multiplexed error-robust fluorescence in situ hybridization (MERFISH), to generate a molecularly defined and spatially resolved cell atlas of the mouse primary motor cortex."
}

source_refs

[
  "paper:paper-bbfc9a9ee9e9"
]

evidence_refs

[
  {
    "ref": "paper:paper-bbfc9a9ee9e9"
  }
]

source_policy

{
  "mode": "public_source_pointer_with_short_context",
  "notes": [
    "Local review repositories are read-only inputs.",
    "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
  ],
  "source_commit_sha": "95e761177f7d2ec565983d3307c14ec238f9677c",
  "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewVIP"
}

Zhang et al. (2021) used MERFISH to generate a molecularly defined and spatiall…

Details

Replication

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