A MERFISH-based spatial cell atlas of mouse primary motor cortex profiling ~300…

A MERFISH-based spatial cell atlas of mouse primary motor cortex profiling ~300,000 cells identifies 95 neuronal and non-neuronal clusters; intratelencephalic (IT) neurons form a continuous gradient along cortical depth in which gene expression of individual cells correlates with their cortical depth, and inhibitory neuronal clusters also adopt laminar organizations.

{
  "n": "~300,000 cells; 95 clusters",
  "doi": "10.1038/s41586-021-03705-x",
  "claim": "A MERFISH-based spatial cell atlas of mouse primary motor cortex profiling ~300,000 cells identifies 95 neuronal and non-neuronal clusters; intratelencephalic (IT) neurons form a continuous gradient along cortical depth in which gene expression of individual cells correlates with their cortical depth, and inhibitory neuronal clusters also adopt laminar organizations.",
  "cite_key": "Zhang2021c",
  "evidence": "MERFISH multiplexed in situ profiling of ~300,000 cells in mouse M1 combined with retrograde labeling to map projection targets.",
  "effect_size": null,
  "text_access": "abstract_only",
  "study_system": "mouse primary motor cortex, MERFISH single-cell spatial transcriptomics (~300,000 cells)",
  "argument_role": "supporting",
  "replication_status": "replication_unknown",
  "claim_source_sentence": "We profiled approximately 300,000 cells in the mouse primary motor cortex and its adjacent areas, identified 95 neuronal and non-neuronal cell clusters, and revealed a complex spatial map in which not only excitatory but also most inhibitory neuronal clusters adopted laminar organizations. Intratelencephalic neurons formed a largely continuous gradient along the cortical depth axis, in which the gene expression of individual cells correlated with their cortical depths.",
  "source_provenance_status": "non_substring_match",
  "replication_evidence_dois": [],
  "effect_size_source_sentence": null
}

[
  "paper:paper-bbfc9a9ee9e9"
]

We profiled approximately 300,000 cells in the mouse primary motor cortex and its adjacent areas, identified 95 neuronal and non-neuronal cell clusters, and revealed a complex spatial map in which not only excitatory but also most inhibitory neuronal clusters adopted laminar organizations. Intratelencephalic neurons formed a largely continuous gradient along the cortical depth axis, in which the gene expression of i...

[
  {
    "ref": "paper:paper-bbfc9a9ee9e9"
  }
]

{
  "mode": "public_source_pointer_with_short_context",
  "notes": [
    "Local review repositories are read-only inputs.",
    "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
  ],
  "source_commit_sha": "79ce062d54a924ce05953ec90aa9d26044d2b48f",
  "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence"
}