MERFISH spatial transcriptomic profiling of ~300,000 cells across mouse primary…

MERFISH spatial transcriptomic profiling of ~300,000 cells across mouse primary motor cortex identified 95 neuronal and non-neuronal clusters and showed that intratelencephalic (IT) neurons form a largely continuous gradient along the cortical depth axis.

{
  "n": 300000,
  "doi": "10.1038/s41586-021-03705-x",
  "claim": "MERFISH spatial transcriptomic profiling of ~300,000 cells across mouse primary motor cortex identified 95 neuronal and non-neuronal clusters and showed that intratelencephalic (IT) neurons form a largely continuous gradient along the cortical depth axis.",
  "cite_key": "Zhang2021c",
  "evidence": "Multiplexed error-robust fluorescence in situ hybridization (MERFISH) imaging of cortical sections in mouse MOp.",
  "effect_size": "~300,000 cells; 95 clusters",
  "text_access": "abstract_only",
  "study_system": "mouse primary motor cortex (MOp) and adjacent areas",
  "argument_role": "supporting",
  "replication_status": "independently_replicated",
  "claim_source_sentence": "We profiled approximately 300,000 cells in the mouse primary motor cortex and its adjacent areas, identified 95 neuronal and non-neuronal cell clusters, and revealed a complex spatial map in which not only excitatory but also most inhibitory neuronal clusters adopted laminar organizations.",
  "source_provenance_status": "non_substring_match",
  "replication_evidence_dois": [
    "10.1038/s41586-021-03950-0",
    "10.1038/s41586-023-06812-z"
  ],
  "effect_size_source_sentence": "We profiled approximately 300,000 cells in the mouse primary motor cortex and its adjacent areas, identified 95 neuronal and non-neuronal cell clusters…"
}

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  "paper:paper-bbfc9a9ee9e9"
]

We profiled approximately 300,000 cells in the mouse primary motor cortex and its adjacent areas, identified 95 neuronal and non-neuronal cell clusters, and revealed a complex spatial map in which not only excitatory but also most inhibitory neuronal clusters adopted laminar organizations.

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