In mouse motor cortex, paired recordings and optogenetic mapping reveal strong…

In mouse motor cortex, paired recordings and optogenetic mapping reveal strong reciprocal connectivity between layer 6 corticothalamic and intratelencephalic neurons but essentially no CT↔PT excitatory connections, with each excitatory pathway accompanied by amplitude-matched disynaptic inhibition.

{
  "n": "mouse M1 slices; sample size not in abstract",
  "doi": "10.1523/jneurosci.4023-14.2015",
  "claim": "In mouse motor cortex, paired recordings and optogenetic mapping reveal strong reciprocal connectivity between layer 6 corticothalamic and intratelencephalic neurons but essentially no CT↔PT excitatory connections, with each excitatory pathway accompanied by amplitude-matched disynaptic inhibition.",
  "cite_key": "Yamawaki2015",
  "evidence": "Paired recordings combined with subcellular ChR2-assisted circuit mapping in mouse M1 slices, applied to CT (Ntsr1), IT, and PT projection-class neurons.",
  "effect_size": "CT↔CT and CT↔IT pathways strong; CT↔PT 'strikingly little'; I/E ratio higher in CT-driven than IT-driven pathways",
  "text_access": "abstract_only",
  "study_system": "mouse forelimb M1, paired recordings + sCRACM",
  "argument_role": "supporting",
  "replication_status": "Consistent with V1/S1 L6 CT excitation of L5 IT and PV interneurons (Crandall 2014; L6CT developmental study 2025)",
  "claim_source_sentence": "Stimulating and recording from CT, intratelencephalic (IT), and pyramidal tract (PT) projection neurons, we found strong CT↔CT and CT↔IT connections; however, CT→IT connections were limited to IT neurons in layer 6, not 5B. There was strikingly little CT↔PT excitatory connectivity.",
  "source_provenance_status": "ok",
  "replication_evidence_dois": [
    "10.1523/JNEUROSCI.1325-14.2014",
    "10.1016/j.celrep.2025.116792"
  ],
  "effect_size_source_sentence": "There was strikingly little CT↔PT excitatory connectivity. ... CT neurons evoked proportionally more inhibition relative to excitation (I/E ratio) than IT neurons."
}

[
  "paper:paper-77ff3f69a352"
]

Stimulating and recording from CT, intratelencephalic (IT), and pyramidal tract (PT) projection neurons, we found strong CT↔CT and CT↔IT connections; however, CT→IT connections were limited to IT neurons in layer 6, not 5B. There was strikingly little CT↔PT excitatory connectivity.

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