Systemically administered CNO does not enter the brain; instead, in vivo conver…

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scope: Rat and rhesus macaque PET imaging; behavioral DREADD experiments
claim_text: Systemically administered CNO does not enter the brain; instead, in vivo conversion of CNO to clozapine is responsible for DREADD activation, with subthreshold systemic clozapine doses sufficient to drive DREADD-mediated behaviors.
section_id: section_11
source_url: https://github.com/AllenNeuralDynamics/ComputationalReviewLoops/blob/0632aae8abc141909207fe91f6349b9e36489c3b/evidence/section_11_evidence_package.json
effect_size: 11
review_repo: ComputationalReviewLoops
section_ref: wiki_page:computationalreviewloops-11
source_kind: review_finding
source_path: evidence/section_11_evidence_package.json
source_span: CNO does not enter the brain after systemic drug injections and shows low affinity for DREADDs. Clozapine, to which CNO rapidly converts in vivo, shows high DREADD affinity and potency.
study_system: Rat and rhesus macaque PET imaging; behavioral DREADD experiments
section_title: Cell-Type-Specific Dissection of Loop Circuits (Opto/Chemo/Tracing)
evidence_summary: PET imaging of [11C]-clozapine binding to DREADD-expressing brain regions in rats and macaques; CNO does not occupy CNS DREADDs but converted clozapine does.
review_bundle_ref: analysis_bundle:ab-d49e54403ef9
replication_status: independently_replicated
review_package_ref: analysis_bundle:ab-d49e54403ef9
source_artifact_ref: wiki_page:computationalreviewloops-11
origin_url: https://github.com/AllenNeuralDynamics/ComputationalReviewLoops/blob/0632aae8abc141909207fe91f6349b9e36489c3b/evidence/section_11_evidence_package.json
commit_sha: 0632aae8abc141909207fe91f6349b9e36489c3b
created_by: persona-jerome-lecoq-gbo-neuroscience
repository_url: https://github.com/AllenNeuralDynamics/ComputationalReviewLoops

Raw fields (4)

raw_fields

{
  "n": 0,
  "doi": "10.1126/science.aan2475",
  "claim": "Systemically administered CNO does not enter the brain; instead, in vivo conversion of CNO to clozapine is responsible for DREADD activation, with subthreshold systemic clozapine doses sufficient to drive DREADD-mediated behaviors.",
  "cite_key": "Gomez2017",
  "evidence": "PET imaging of [11C]-clozapine binding to DREADD-expressing brain regions in rats and macaques; CNO does not occupy CNS DREADDs but converted clozapine does.",
  "effect_size": "11",
  "text_access": "fulltext",
  "study_system": "Rat and rhesus macaque PET imaging; behavioral DREADD experiments",
  "source_cluster_id": "cluster_10",
  "replication_status": "independently_replicated",
  "claim_source_sentence": "CNO does not enter the brain after systemic drug injections and shows low affinity for DREADDs. Clozapine, to which CNO rapidly converts in vivo, shows high DREADD affinity and potency.",
  "replication_evidence_dois": [
    "10.1016/j.neuron.2016.01.040"
  ],
  "effect_size_source_sentence": "PET imaging of [11C]-clozapine binding to DREADD-expressing brain regions in rats and macaques; CNO does not occupy CNS DREADDs but converted clozapine does."
}

source_refs

[
  "paper:paper-faa5cef87aeb"
]

evidence_refs

[
  {
    "ref": "paper:paper-faa5cef87aeb"
  }
]

source_policy

{
  "mode": "public_source_pointer_with_short_context",
  "notes": [
    "Local review repositories are read-only inputs.",
    "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
  ],
  "source_commit_sha": "0632aae8abc141909207fe91f6349b9e36489c3b",
  "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewLoops"
}

Systemically administered CNO does not enter the brain; instead, in vivo conver…

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