In vivo two-photon-targeted paired recordings between mouse layer 2 barrel-cort…

In vivo two-photon-targeted paired recordings between mouse layer 2 barrel-cortex pyramidal neurons during the hyperpolarized down-state reveal a sparsely connected network (~6.7% connection probability) with overrepresentation of bidirectional pairs, small unitary EPSPs (<0.5 mV), and 22% failure rate, providing the first in vivo benchmark of L2/3 recurrent E→E translaminar connectivity.

{
  "n": "in vivo two-photon multi-patch, n pairs in main text",
  "doi": "10.1016/j.celrep.2015.11.011",
  "claim": "In vivo two-photon-targeted paired recordings between mouse layer 2 barrel-cortex pyramidal neurons during the hyperpolarized down-state reveal a sparsely connected network (~6.7% connection probability) with overrepresentation of bidirectional pairs, small unitary EPSPs (<0.5 mV), and 22% failure rate, providing the first in vivo benchmark of L2/3 recurrent E→E translaminar connectivity.",
  "cite_key": "Jouhanneau2015",
  "evidence": "Two-photon-targeted multi-cell (2-4) whole-cell recordings of neighbouring L2 pyramidal cells in mouse barrel cortex in vivo.",
  "effect_size": "Connection probability 6.7%; failure rate 22%; CV = 0.74; mean PPR = 1.15; unitary EPSP <0.5 mV majority",
  "text_access": "fulltext",
  "study_system": "mouse barrel L2 in vivo whole-cell",
  "argument_role": "supporting",
  "replication_status": "consistent with sparse L2/3 recurrent connectivity (Lefort 2009; Avermann 2012)",
  "claim_source_sentence": "We made multiple (two to four) two-photon targeted whole-cell recordings from neighboring layer 2 mouse somatosensory barrel cortex pyramidal neurons in vivo... pyramidal neurons form a sparsely connected (6.7% connectivity) network with an overrepresentation of bidirectional connections. The majority of unitary excitatory postsynaptic potentials were small in amplitude (<0.5 mV), with a small minority >1 mV. The coefficient of variation (CV = 0.74) could largely be explained by the presence of synaptic failures (22%).",
  "source_provenance_status": "ok",
  "replication_evidence_dois": [
    "10.1016/j.neuron.2008.12.020"
  ],
  "effect_size_source_sentence": "pyramidal neurons form a sparsely connected (6.7% connectivity) network with an overrepresentation of bidirectional connections. ... CV = 0.74... synaptic failures (22%)."
}

[
  "paper:paper-6d3d01cb91a6"
]

We made multiple (two to four) two-photon targeted whole-cell recordings from neighboring layer 2 mouse somatosensory barrel cortex pyramidal neurons in vivo... pyramidal neurons form a sparsely connected (6.7% connectivity) network with an overrepresentation of bidirectional connections. The majority of unitary excitatory postsynaptic potentials were small in amplitude (<0.5 mV), with a small minority >1 mV. The co...

[
  {
    "ref": "paper:paper-6d3d01cb91a6"
  }
]

{
  "mode": "public_source_pointer_with_short_context",
  "notes": [
    "Local review repositories are read-only inputs.",
    "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
  ],
  "source_commit_sha": "79ce062d54a924ce05953ec90aa9d26044d2b48f",
  "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence"
}