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{
  "pmid": "35545715",
  "doi": "10.1038/s41592-022-01468-5",
  "abstract": "Light-sheet microscopy has emerged as the preferred means for high-throughput volumetric imaging of cleared tissues. However, there is a need for a flexible system that can address imaging applications with varied requirements in terms of resolution, sample size, tissue-clearing protocol, and transparent sample-holder material. Here, we present a 'hybrid' system that combines a unique non-orthogonal dual-objective and conventional (orthogonal) open-top light-sheet (OTLS) architecture for versatile multi-scale volumetric imaging. We demonstrate efficient screening and targeted sub-micrometer imaging of sparse axons within an intact, cleared mouse brain. The same system enables high-throughput automated imaging of multiple specimens, as spotlighted by a quantitative multi-scale analysis of brain metastases. Compared with existing academic and commercial light-sheet microscopy systems, our hybrid OTLS system provides a unique combination of versatility and performance necessary to satisfy the diverse requirements of a growing number of cleared-tissue imaging applications.",
  "journal": "Nature Methods",
  "year": 2022,
  "authors": "Adam K. Glaser, Kevin W. Bishop, Lindsey A. Barner, Etsuo A. Susaki, Shimpei I. Kubota, Gan Gao et al.",
  "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/9214839",
  "pmc_id": "",
  "external_ids": {
    "doi": "10.1038/s41592-022-01468-5",
    "pmid": "35545715",
    "pmcid": "",
    "openalex": "W4280562732"
  },
  "citation_count": 167
}