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{
  "pmid": "36922596",
  "doi": "10.1038/s41586-023-05828-9",
  "abstract": "Calcium imaging with protein-based indicators<sup>1,2</sup> is widely used to follow neural activity in intact nervous systems, but current protein sensors report neural activity at timescales much slower than electrical signalling and are limited by trade-offs between sensitivity and kinetics. Here we used large-scale screening and structure-guided mutagenesis to develop and optimize several fast and sensitive GCaMP-type indicators<sup>3-8</sup>. The resulting 'jGCaMP8' sensors, based on the calcium-binding protein calmodulin and a fragment of endothelial nitric oxide synthase, have ultra-fast kinetics (half-rise times of 2 ms) and the highest sensitivity for neural activity reported for a protein-based calcium sensor. jGCaMP8 sensors will allow tracking of large populations of neurons on timescales relevant to neural computation.",
  "journal": "Nature",
  "year": 2023,
  "authors": "Yan Zhang, Márton Rózsa, Yajie Liang, Daniel Bushey, Ziqiang Wei, Jihong Zheng et al.",
  "url": "https://www.nature.com/articles/s41586-023-05828-9.pdf",
  "pmc_id": "",
  "external_ids": {
    "doi": "10.1038/s41586-023-05828-9",
    "pmid": "36922596",
    "pmcid": "",
    "openalex": "W4324344199"
  },
  "citation_count": 769
}