Version history

{
  "pmid": "28079019",
  "doi": "10.1101/097188",
  "abstract": "We describe Cleavage Under Targets and Release Using Nuclease (CUT&RUN), a chromatin profiling strategy in which antibody-targeted controlled cleavage by micrococcal nuclease releases specific protein-DNA complexes into the supernatant for paired-end DNA sequencing. Unlike Chromatin Immunoprecipitation (ChIP), which fragments and solubilizes total chromatin, CUT&RUN is performed in situ, allowing for both quantitative high-resolution chromatin mapping and probing of the local chromatin environment. When applied to yeast and human nuclei, CUT&RUN yielded precise transcription factor profiles while avoiding cross-linking and solubilization issues. CUT&RUN is simple to perform and is inherently robust, with extremely low backgrounds requiring only ~1/10th the sequencing depth as ChIP, making CUT&RUN especially cost-effective for transcription factor and chromatin profiling. When used in conjunction with native ChIP-seq and applied to human CTCF, CUT&RUN mapped directional long range contacts at high resolution. We conclude that in situ mapping of protein-DNA interactions by CUT&RUN is an attractive alternative to ChIP-seq.",
  "journal": "bioRxiv",
  "year": 2016,
  "authors": "P. Skene, S. Henikoff",
  "mesh_terms": "",
  "url": "https://doi.org/10.7554/elife.21856",
  "pmc_id": "PMC5310842",
  "external_ids": {
    "doi": "10.1101/097188",
    "mag": "2562443513",
    "pmid": "28079019",
    "orcid": "https://orcid.org/0000-0001-8965-5326",
    "pmcid": "PMC5310842",
    "s2_id": "17688529",
    "pmc_id": "PMC5310842",
    "openalex": "https://openalex.org/W2562443513",
    "pmc_lookup_at": "2026-04-27T00:26:59.845557+00:00",
    "openalex_author": "https://openalex.org/A5072662718",
    "pmc_lookup_source": "pubmed_efetch",
    "scientist_author_slugs": [
      "pete-skene"
    ],
    "scientist_author_orcids": [
      "0000-0001-8965-5326"
    ]
  },
  "citation_count": 1872
}