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{
  "pmid": "34650269",
  "doi": "10.1038/s41587-021-01025-z",
  "abstract": "Current methods to delete genomic sequences are based on clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 and pairs of single-guide RNAs (sgRNAs), but can be inefficient and imprecise, with errors including small indels as well as unintended large deletions and more complex rearrangements. In the present study, we describe a prime editing-based method, PRIME-Del, which induces a deletion using a pair of prime editing sgRNAs (pegRNAs) that target opposite DNA strands, programming not only the sites that are nicked but also the outcome of the repair. PRIME-Del achieves markedly higher precision than CRISPR-Cas9 and sgRNA pairs in programming deletions up to 10 kb, with 1-30% editing efficiency. PRIME-Del can also be used to couple genomic deletions with short insertions, enabling deletions with junctions that do not fall at protospacer-adjacent motif sites. Finally, extended expression of prime editing components can substantially enhance efficiency without compromising precision. We anticipate that PRIME-Del will be broadly useful for precise, flexible programming of genomic deletions, epitope tagging and, potentially, programming genomic rearrangements.",
  "journal": "Nature Biotechnology",
  "year": 2021,
  "authors": "Junhong Choi, Wei Chen, Chase C. Suiter, Choli Lee, Florence M. Chardon et al.",
  "external_ids": {
    "doi": "10.1038/s41587-021-01025-z",
    "pmid": "34650269"
  },
  "citation_count": 235
}