TREM2 Ectodomain Variant — Stability Optimized

MEPLRGLLLLLLPLLGLLGPRVTEAQVTYLECSTPGYHIAAGWAMLSCFVKELTHRRRSLQMDSYRAATMSSKKMLETQGDLEKIEELEEMVVPAEPEGQDTEAQVTYLECSTPGYHIAAGWAMLSCFVKELTHRRRSLQMDSYRAATMSSKKMLETQGDLEKIEELEEMVVPAEPEGQDTEAQVTYLECSTPGYHIAAGWAMLSCFVKELTHRRRSLQMDSYRAATMSSKKMLETQGDLERDHPG

{
  "method": "rosetta_relax",
  "sequence": "MEPLRGLLLLLLPLLGLLGPRVTEAQVTYLECSTPGYHIAAGWAMLSCFVKELTHRRRSLQMDSYRAATMSSKKMLETQGDLEKIEELEEMVVPAEPEGQDTEAQVTYLECSTPGYHIAAGWAMLSCFVKELTHRRRSLQMDSYRAATMSSKKMLETQGDLEKIEELEEMVVPAEPEGQDTEAQVTYLECSTPGYHIAAGWAMLSCFVKELTHRRRSLQMDSYRAATMSSKKMLETQGDLERDHPG",
  "mutations": [
    "T96S",
    "K186R",
    "A192V"
  ],
  "uniprot_id": "Q9NZC2",
  "delta_G_kcal": -2.3,
  "binding_affinity": {
    "kd_nm": 780,
    "target": "Aβ oligomers"
  },
  "design_rationale": "Rosetta-guided mutations to improve thermostability without disrupting binding interface",
  "predicted_stability": 0.91
}