TREM2 Ectodomain Variant — Binding Optimized

MEPLRGLLLLLLPLLGLLGPRVTEAQVTYLECSTPGYHIAAGWAMLSCFVKELTHRRRSLQMDSYRAATMSSKKMLETQGDLEKIEELEEMVVPAEPEGQDTEAQVTYLECSTPGYHIAAGWAMLSCFVKELTHRRRSLQMDSYRAATMSSKKMLETQGDLEKIEELEEMVVPAEPEGQDTEAQVTYLECSTPGYHIAAGWAMLSCFVKELTHRRRSLQMDSYRAATMSSKKMLETQGDLERDHPG

{
  "method": "rosetta_interface_design",
  "sequence": "MEPLRGLLLLLLPLLGLLGPRVTEAQVTYLECSTPGYHIAAGWAMLSCFVKELTHRRRSLQMDSYRAATMSSKKMLETQGDLEKIEELEEMVVPAEPEGQDTEAQVTYLECSTPGYHIAAGWAMLSCFVKELTHRRRSLQMDSYRAATMSSKKMLETQGDLEKIEELEEMVVPAEPEGQDTEAQVTYLECSTPGYHIAAGWAMLSCFVKELTHRRRSLQMDSYRAATMSSKKMLETQGDLERDHPG",
  "mutations": [
    "T96S",
    "K186R",
    "A192V",
    "H157Y",
    "R62W"
  ],
  "uniprot_id": "Q9NZC2",
  "delta_G_kcal": -1.8,
  "binding_affinity": {
    "kd_nm": 120,
    "target": "Aβ oligomers"
  },
  "design_rationale": "Interface residue optimization for enhanced Aβ oligomer recognition. Slight stability trade-off accepted for 6.5x binding improvement.",
  "predicted_stability": 0.88
}