Dorsal forebrain organoid IN composition shifts over development: LGE-like INs…

Dorsal forebrain organoid IN composition shifts over development: LGE-like INs are present at 8 weeks but are gradually replaced by CGE-like INs by 15 weeks, when they constitute the majority of organoid INs. CGE-like INs map to two cardinal subtypes: NR2F2+/PROX1+ (CGE-restricted) and MEIS2+/PAX6+ (CGE+LGE), with predicted fates as cortical INs, olfactory bulb INs, white-matter interstitial neurons, and eccentric striatal projection neurons.

{
  "n": null,
  "doi": "10.1016/j.stem.2023.08.009",
  "claim": "Dorsal forebrain organoid IN composition shifts over development: LGE-like INs are present at 8 weeks but are gradually replaced by CGE-like INs by 15 weeks, when they constitute the majority of organoid INs. CGE-like INs map to two cardinal subtypes: NR2F2+/PROX1+ (CGE-restricted) and MEIS2+/PAX6+ (CGE+LGE), with predicted fates as cortical INs, olfactory bulb INs, white-matter interstitial neurons, and eccentric striatal projection neurons.",
  "title": null,
  "cite_key": "Andrews2023",
  "evidence": "Dorsal forebrain organoid scRNA-seq across 8, 11, 15 weeks; correlation to primary human GE-derived INs.",
  "effect_size": null,
  "figure_data": null,
  "text_access": "fulltext",
  "key_entities": [
    "dorsal organoid",
    "CGE-like",
    "LGE-like",
    "NR2F2",
    "PROX1",
    "MEIS2",
    "PAX6",
    "8 weeks",
    "15 weeks",
    "human",
    "temporal"
  ],
  "study_system": "human dorsal forebrain organoids 8–15 weeks",
  "_source_cluster": "cluster_02_development_lineage",
  "replication_status": "single_study",
  "_source_cluster_index": 129,
  "claim_source_sentence": "We observed that LGE-like INs were present at 8 weeks in organoids but were gradually replaced by CGE-like INs over time. By 15 weeks, the majority of INs in organoids were CGE-like. We found that CGE-like neurons mostly resembled two IN subtypes: NR2F2+/PROX1+ INs found in CGE and MEIS2+/PAX6+ INs found in both CGE and LGE.",
  "replication_evidence_dois": [],
  "effect_size_source_sentence": null
}

[
  "paper:paper-14ad138608e3"
]

We observed that LGE-like INs were present at 8 weeks in organoids but were gradually replaced by CGE-like INs over time. By 15 weeks, the majority of INs in organoids were CGE-like. We found that CGE-like neurons mostly resembled two IN subtypes: NR2F2+/PROX1+ INs found in CGE and MEIS2+/PAX6+ INs found in both CGE and LGE.

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