RNA sequencing of half a million single cells across the entire mouse nervous s…

RNA sequencing of half a million single cells across the entire mouse nervous system produced a hierarchical, data-driven cell-type taxonomy in which neurons are grouped by developmental anatomical units and neurotransmitter/neuropeptide expression, with seven regionally restricted astrocyte types respecting developmental boundaries while oligodendrocytes show secondary regional diversification.

{
  "n": 500000,
  "doi": "10.1016/j.cell.2018.06.021",
  "claim": "RNA sequencing of half a million single cells across the entire mouse nervous system produced a hierarchical, data-driven cell-type taxonomy in which neurons are grouped by developmental anatomical units and neurotransmitter/neuropeptide expression, with seven regionally restricted astrocyte types respecting developmental boundaries while oligodendrocytes show secondary regional diversification.",
  "cite_key": "Zeisel2018",
  "evidence": "Single-cell RNA sequencing of ~500,000 cells from the adult mouse nervous system, integrated across 19 dissected regions, produced a unified taxonomy of cell types and revealed regional restriction of astrocyte subtypes versus secondary diversification of oligodendrocytes.",
  "effect_size": "~500,000 single cells; 7 distinct astrocyte types",
  "text_access": "fulltext",
  "study_system": "adult mouse nervous system (CNS+PNS); ~500,000 single cells",
  "source_cluster_id": "cluster_14",
  "replication_status": "independently_replicated",
  "claim_source_sentence": "Here, we used RNA sequencing of half a million single cells to create a detailed census of cell types in the mouse nervous system.",
  "replication_evidence_dois": [
    "10.1038/s41586-021-03465-8",
    "10.1126/science.aar4237"
  ],
  "effect_size_source_sentence": "We discovered seven distinct, regionally restricted astrocyte types that obeyed developmental boundaries and correlated with the spatial distribution of key glutamate and glycine neurotransmitters."
}

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]

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Single-cell RNA sequencing of ~500,000 cells from the adult mouse nervous system, integrated across 19 dissected regions, produced a unified taxonomy of cell types and revealed regional restriction of astrocyte subtypes versus secondary diversification of oligodendrocytes.