BARseq, an in-situ-sequencing-based barcoded projection mapping technique, mapp…

BARseq, an in-situ-sequencing-based barcoded projection mapping technique, mapped the projections of 3,579 spatially resolved neurons in mouse auditory cortex to 11 areas, confirming laminar segregation of IT, PT-like, and CT projection classes and revealing a projection type restricted almost exclusively to transcriptionally defined IT subtypes.

{
  "n": 3579,
  "doi": "10.1016/j.cell.2019.09.023",
  "claim": "BARseq, an in-situ-sequencing-based barcoded projection mapping technique, mapped the projections of 3,579 spatially resolved neurons in mouse auditory cortex to 11 areas, confirming laminar segregation of IT, PT-like, and CT projection classes and revealing a projection type restricted almost exclusively to transcriptionally defined IT subtypes.",
  "cite_key": "Chen2019",
  "evidence": "In-situ sequencing of RNA barcodes injected via Sindbis virus to read out single-neuron projection patterns combined with gene-expression readout in mouse auditory cortex.",
  "effect_size": "3,579 neurons; 11 target areas; 3 major projection classes (IT, PT-like, CT)",
  "text_access": "fulltext",
  "study_system": "mouse (BARseq in auditory cortex)",
  "source_cluster_id": "cluster_03",
  "replication_status": "replication_unknown",
  "claim_source_sentence": "Consistent with the fact that PT-l but not CT neurons project to the striatum, only 8 / 250 (3.2%) putative CT neurons sequenced in situ projected to the caudal striatum, whereas 59 / 245 (24%) putative PT-l neurons sequenced in situ projected to the caudal striatum.",
  "replication_evidence_dois": [],
  "effect_size_source_sentence": "Mapping the projections to 11 areas of 3,579 neurons in mouse auditory cortex using BARseq confirmed the laminar organization of the three top classes (intratelencephalic [IT], pyramidal tract-like [PT-like], and corticothalamic [CT]) of projection neurons."
}

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Consistent with the fact that PT-l but not CT neurons project to the striatum, only 8 / 250 (3.2%) putative CT neurons sequenced in situ projected to the caudal striatum, whereas 59 / 245 (24%) putative PT-l neurons sequenced in situ projected to the caudal striatum.

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