A two-photon block-face fluorescence imaging platform integrated with a vibrato…

{
  "n": null,
  "doi": "10.7554/elife.10566",
  "claim": "A two-photon block-face fluorescence imaging platform integrated with a vibratome enables whole-brain visualization and reconstruction of complete long-range axonal arbors at sub-micron resolution.",
  "cite_key": "Economo2016",
  "evidence": "Demonstrated reconstruction of axonal arbors of multiple mouse motor-cortex neurons across an entire brain, with axon detection down to ~100 nm diameter.",
  "effect_size": "Axon diameters as small as 100 nm visualized; arbors spanning many millimeters reconstructed",
  "text_access": "fulltext",
  "study_system": "mouse (whole-brain serial two-photon tomography)",
  "source_cluster_id": "cluster_03",
  "replication_status": "replication_unknown",
  "claim_source_sentence": "We have developed an imaging system for whole-brain, high-resolution fluorescence imaging and demonstrate that this approach can be used to trace individual axonal fibers and fine axon collater- als across the brain to their termini ( Video 2 ) and thereby map the long-distance projections of sin- gle neurons ( Figure 6 ).",
  "replication_evidence_dois": [],
  "effect_size_source_sentence": "However, the arbors of very few long-range projection neurons have been reconstructed in their entirety, as axons with diameters as small as 100 nm arborize in target regions dispersed over many millimeters of tissue."
}

[
  "paper:b99d7c4a-9270-42c0-b083-2109f9c1eabf"
]

We have developed an imaging system for whole-brain, high-resolution fluorescence imaging and demonstrate that this approach can be used to trace individual axonal fibers and fine axon collater- als across the brain to their termini ( Video 2 ) and thereby map the long-distance projections of sin- gle neurons ( Figure 6 ).

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