Mouse M2 layer-5 intratelencephalic (IT) Tlx3 neurons innervate L1 versus L2/3…

Mouse M2 layer-5 intratelencephalic (IT) Tlx3 neurons innervate L1 versus L2/3 of target cortices in a target-area-specific manner: uniform L1/L2/3 in local M2 but preferentially L1 in ipsilateral M1, S1 and AUD, with similar preferences in contralateral hemispheres.

{
  "n": 3,
  "doi": "10.1093/cercor/bhac052",
  "claim": "Mouse M2 layer-5 intratelencephalic (IT) Tlx3 neurons innervate L1 versus L2/3 of target cortices in a target-area-specific manner: uniform L1/L2/3 in local M2 but preferentially L1 in ipsilateral M1, S1 and AUD, with similar preferences in contralateral hemispheres.",
  "cite_key": "Im2022",
  "evidence": "Anterograde tracing with synaptophysin-EGFP from M2 L5 Tlx3-Cre IT neurons; manual puncta counting across L1 and matched-thickness L2/3 (128 ± 26 μm) in multiple target cortical regions; complemented with MouseLight single-axon reconstructions.",
  "effect_size": "L1-bias index 0.53–0.66 in distal cortical targets vs. ~0 in local M2; paired-test contra vs ipsi non-significant.",
  "text_access": "fulltext",
  "study_system": "adult mouse cortex, anterograde axonal tracing of M2 L5 IT Tlx3-Cre neurons + retrograde labeling + MouseLight single-axon reconstructions",
  "argument_role": "supporting",
  "replication_status": "independently_replicated",
  "claim_source_sentence": "M2-Tlx3 cells uniformly innervated L1 and L2/3 in local M2 (index = −0.0022 ± 0.027, 3 mice, = 0.90, one sample-test, hypothetical value = 0), but preferentially L1 in ipsilateral M1 (index = 0.53 ± 0.15; = 0.024), ipsilateral S1 (index = 0.60 ± 0.15; = 0.020), ipsilateral AUD (index = 0.66 ± 0.16; = 0.",
  "source_provenance_status": "ok",
  "replication_evidence_dois": [
    "10.1038/nn1891",
    "10.1038/nature07709"
  ],
  "effect_size_source_sentence": "M2-Tlx3 cells uniformly innervated L1 and L2/3 in local M2 (index = −0.0022 ± 0.027, 3 mice, = 0.90, one sample-test, hypothetical value = 0), but preferentially L1 in ipsilateral M1 (index = 0.53 ± 0.15; = 0.024), ipsilateral S1 (index = 0.60 ± 0.15; = 0.020), ipsilateral AUD (index = 0.66 ± 0.16; = 0."
}

[
  "paper:paper-e55711a2961a"
]

M2-Tlx3 cells uniformly innervated L1 and L2/3 in local M2 (index = −0.0022 ± 0.027, 3 mice, = 0.90, one sample-test, hypothetical value = 0), but preferentially L1 in ipsilateral M1 (index = 0.53 ± 0.15; = 0.024), ipsilateral S1 (index = 0.60 ± 0.15; = 0.020), ipsilateral AUD (index = 0.66 ± 0.16; = 0.

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Anterograde tracing with synaptophysin-EGFP from M2 L5 Tlx3-Cre IT neurons; manual puncta counting across L1 and matched-thickness L2/3 (128 ± 26 μm) in multiple target cortical regions; complemented with MouseLight single-axon reconstructions.