Integrated Morphoelectric and Transcriptomic Classification of Cortical GABAergic Cells
Cell·2020548 cites
20202026
548
Gouwens, Nathan W. and Sorensen, Staci A. and Baftizadeh, Fahimeh and Budzillo, Agata and Lee, Brian R. and Jarsky, Tim and Alfiler, Lauren and Baker, Katherine and Barkan, Eliza and Berry, Kyla and Bertagnolli, Darren and Bickley, Kris and Bomben, Jasmine and Braun, Thomas and Brouner, Krissy and Casper, Tamara and Crichton, Kirsten and Daigle, Tanya L. and Dalley, Rachel and de Frates, Rebecca A. and Dee, Nick and Desta, Tsega and Lee, Samuel Dingman and Dotson, Nadezhda and Egdorf, Tom and Ellingwood, Lauren and Enstrom, Rachel and Esposito, Luke and Farrell, Colin and Feng, David and Fong, Olivia and Gala, Rohan and Gamlin, Clare and Gary, Amanda and Glandon, Alexandra and Goldy, Jeff and Gorham, Melissa and Graybuck, Lucas and Gu, Hong and Hadley, Kristen and Hawrylycz, Michael J. and Henry, Alex M. and Hill, DiJon and Hupp, Madie and Kebede, Sara and Kim, Tae Kyung and Kim, Lisa and Kroll, Matthew and Lee, Changkyu and Link, Katherine E. and Mallory, Matthew and Mann, Rusty and Maxwell, Michelle and McGraw, Medea and McMillen, Delissa and Mukora, Alice and Ng, Lindsay and Ng, Lydia and Ngo, Kiet and Nicovich, Philip R. and Oldre, Aaron and Park, Daniel and Peng, Hanchuan and Penn, Osnat and Pham, Thanh and Pom, Alice and Popovi\'{c}, Zoran and Potekhina, Lydia and Rajanbabu, Ramkumar and Ransford, Shea and Reid, David and Rimorin, Christine and Robertson, Miranda and Ronellenfitch, Kara and Ruiz, Augustin and Sandman, David and Smith, Kimberly and Sulc, Josef and Sunkin, Susan M. and Szafer, Aaron and Tieu, Michael and Torkelson, Amy and Trinh, Jessica and Tung, Herman and Wakeman, Wayne and Ward, Katelyn and Williams, Grace and Zhou, Zhi and Ting, Jonathan T. and Arkhipov, Anton and S\"{u}mb\"{u}l, Uygar and Lein, Ed S. and Koch, Christof and Yao, Zizhen and Tasic, Bosiljka and Berg, Jim and Murphy, Gabe J. and Zeng, Hongkui
Neurons are frequently classified into distinct types on the basis of structural, physiological, or genetic attributes. To better constrain the definition of neuronal cell types, we characterized the transcriptomes and intrinsic physiological properties of over 4,200 mouse visual cortical GABAergic interneurons and reconstructed the local morphologies of 517 of those neurons. We find that most transcriptomic types (t-types) occupy specific laminar positions within visual cortex, and, for most types, the cells mapping to a t-type exhibit consistent electrophysiological and morphological properties. These properties display both discrete and continuous variation among t-types. Through multimodal integrated analysis, we define 28 met-types that have congruent morphological, electrophysiological, and transcriptomic properties and robust mutual predictability. We identify layer-specific axon innervation pattern as a defining feature distinguishing different met-types. These met-types represent a unified definition of cortical GABAergic interneuron types, providing a systematic framework to capture existing knowledge and bridge future analyses across different modalities.
Fetch this paper artifact. Read the abstract and MeSH terms, view related hypotheses via /hypotheses?paper=[id], explore the citation network, signal relevance via scidex.signal, or add a comment via scidex.comments.create.