Patch-seq characterization of 4,200+ mouse visual-cortical GABAergic interneuro…

Patch-seq characterization of 4,200+ mouse visual-cortical GABAergic interneurons with morphological reconstructions of 517 cells defines 28 morpho-electric-transcriptomic (MET) types, providing a unified definition of cortical inhibitory cell types in which most transcriptomic types occupy specific layers and show consistent intrinsic physiology.

{
  "n": 4200,
  "doi": "10.1016/j.cell.2020.09.057",
  "claim": "Patch-seq characterization of 4,200+ mouse visual-cortical GABAergic interneurons with morphological reconstructions of 517 cells defines 28 morpho-electric-transcriptomic (MET) types, providing a unified definition of cortical inhibitory cell types in which most transcriptomic types occupy specific layers and show consistent intrinsic physiology.",
  "cite_key": "Gouwens2020",
  "evidence": "Foundational large-scale Patch-seq dataset for mouse visual cortex inhibitory cells, providing reference cell-type taxonomy used throughout cluster_14 discussion of cortical types.",
  "effect_size": "4,200+ Patch-seq cells; 517 morphologies reconstructed; 28 MET-types defined",
  "text_access": "abstract_only",
  "study_system": "Mouse visual cortex; Patch-seq of GABAergic interneurons",
  "argument_role": "supporting",
  "replication_status": "replicated",
  "claim_source_sentence": "To better constrain the definition of neuronal cell types, we characterized the transcriptomes and intrinsic physiological properties of over 4,200 mouse visual cortical GABAergic interneurons and reconstructed the local morphologies of 517 of those neurons.",
  "source_provenance_status": "non_substring_match",
  "replication_evidence_dois": [
    "10.7554/elife.65482",
    "10.1126/science.adf6484"
  ],
  "effect_size_source_sentence": "Through multimodal integrated analysis, we define 28 met-types that have congruent morphological, electrophysiological, and transcriptomic properties and robust mutual predictability."
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To better constrain the definition of neuronal cell types, we characterized the transcriptomes and intrinsic physiological properties of over 4,200 mouse visual cortical GABAergic interneurons and reconstructed the local morphologies of 517 of those neurons.

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15. Methodological limits and emerging tools — what current mouse-cortex tools cannot yet measure about E→E recurrence (subthreshold network activity, fast plasticity in vivo, millimetre-scale dynamic connectomes), and what is on the near horizon

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