Patch-seq of >1,300 adult mouse primary motor cortex neurons morpho-electricall…

Patch-seq of >1,300 adult mouse primary motor cortex neurons morpho-electrically annotated nearly all transcriptomic types, finding broad transcriptomic families (Vip, Pvalb, Sst, etc.) had non-overlapping morpho-electric phenotypes while individual transcriptomic types within a family were not well separated in morpho-electric space.

{
  "n": 1300,
  "doi": "10.1038/s41586-020-2907-3",
  "claim": "Patch-seq of >1,300 adult mouse primary motor cortex neurons morpho-electrically annotated nearly all transcriptomic types, finding broad transcriptomic families (Vip, Pvalb, Sst, etc.) had non-overlapping morpho-electric phenotypes while individual transcriptomic types within a family were not well separated in morpho-electric space.",
  "cite_key": "Scala2021",
  "evidence": "Patch-seq combining whole-cell patch-clamp, biocytin filling, and full-transcriptome single-cell sequencing of >1,300 cortical neurons in adult mouse M1.",
  "effect_size": "1,300+ neurons; broad transcriptomic families separable in morpho-electric space but fine within-family types overlap",
  "text_access": "abstract_only",
  "study_system": "adult mouse primary motor cortex",
  "argument_role": "supporting",
  "replication_status": "independently_replicated",
  "claim_source_sentence": "Here we used Patch-seqto combine patch-clamp recording, biocytin staining, and single-cell RNA sequencing of more than 1,300 neurons in adult mouse primary motor cortex, providing a morpho-electric annotation of almost all transcriptomically defined neural cell types.",
  "source_provenance_status": "non_substring_match",
  "replication_evidence_dois": [
    "10.1101/2023.11.25.568393",
    "10.1038/s41586-021-03950-0"
  ],
  "effect_size_source_sentence": "…although broad families of transcriptomic types (those expressing Vip, Pvalb, Sst and so on) had distinct and essentially non-overlapping morpho-electric phenotypes, individual transcriptomic types within the same family were not well separated in the morpho-electric space."
}

[
  "paper:paper-be132b319290"
]

Here we used Patch-seqto combine patch-clamp recording, biocytin staining, and single-cell RNA sequencing of more than 1,300 neurons in adult mouse primary motor cortex, providing a morpho-electric annotation of almost all transcriptomically defined neural cell types.

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