- claim_text
Fuzik et al. (2016) developed an independent Patch-seq variant combining whole-cell patch-clamp recordings with full-transcriptome single-cell RNA-seq from neocortical pyramidal cells and interneurons, establishing a method now widely used for VIP morphoelectric-transcriptomic typing.
- raw_fields
{
"n": null,
"doi": "10.1038/nbt.3443",
"claim": "Fuzik et al. (2016) developed an independent Patch-seq variant combining whole-cell patch-clamp recordings with full-transcriptome single-cell RNA-seq from neocortical pyramidal cells and interneurons, establishing a method now widely used for VIP morphoelectric-transcriptomic typing.",
"title": null,
"cite_key": "Fuzik2016",
"evidence": "Patch-seq workflow: patch-clamp ephys → aspirate cytoplasm → STRT-seq RNA-seq → cluster cells transcriptomically; published concurrently with Cadwell et al. 2016 establishing two independent multimodal workflows.",
"effect_size": null,
"text_access": "fulltext",
"study_system": "mouse neocortex slices; Patch-seq method paper",
"target_section": "section_11",
"_source_cluster": "cluster_01_molecular_taxonomy",
"replication_status": "replicated",
"_source_cluster_index": 27,
"claim_source_sentence": "Here, we present a method to obtain full transcriptome data from single neocortical pyramidal cells and interneurons after whole-cell patch-clamp recordings in mouse brain slices."
}- source_refs
[
"paper:paper-fd924ebdd0c4"
]
- evidence_refs
[
{
"ref": "paper:paper-fd924ebdd0c4"
}
]- source_policy
{
"mode": "public_source_pointer_with_short_context",
"notes": [
"Local review repositories are read-only inputs.",
"SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
],
"source_commit_sha": "95e761177f7d2ec565983d3307c14ec238f9677c",
"source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewVIP"
}