Matched single-nucleus and single-cell RNA-seq from mouse visual cortex showed…

Matched single-nucleus and single-cell RNA-seq from mouse visual cortex showed that, despite ~11,000 genes detected per whole cell vs ~7,000 per nucleus, closely related neuronal types can be similarly discriminated by snRNA-seq when intronic reads are retained.

{
  "n": null,
  "doi": "10.1371/journal.pone.0209648",
  "claim": "Matched single-nucleus and single-cell RNA-seq from mouse visual cortex showed that, despite ~11,000 genes detected per whole cell vs ~7,000 per nucleus, closely related neuronal types can be similarly discriminated by snRNA-seq when intronic reads are retained.",
  "title": null,
  "cite_key": "Bakken2018",
  "evidence": "Matched snRNA-seq and scRNA-seq from mouse V1; comparison of cell-type discrimination power and per-cell gene detection.",
  "effect_size": null,
  "text_access": "abstract_only",
  "study_system": "mouse V1 paired sn/scRNA-seq benchmark",
  "target_section": "section_02",
  "_source_cluster": "cluster_01_molecular_taxonomy",
  "replication_status": "single_study",
  "_source_cluster_index": 53,
  "claim_source_sentence": "Although more transcripts are detected in individual whole cells (~11,000 genes) than nuclei (~7,000 genes), we demonstrate that closely related neuronal cell types can be similarly discriminated with both methods if intronic sequences are included in snRNA-seq analysis."
}

[
  "paper:paper-pm-30586455"
]

Although more transcripts are detected in individual whole cells (~11,000 genes) than nuclei (~7,000 genes), we demonstrate that closely related neuronal cell types can be similarly discriminated with both methods if intronic sequences are included in snRNA-seq analysis.

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