Cortical 5-HT3A receptor-expressing interneurons are predominantly generated in…

Cortical 5-HT3A receptor-expressing interneurons are predominantly generated in the caudal ganglionic eminence; in utero CGE grafts of E14 Htr3a-GFP+ cells reproduce the supragranular laminar distribution and neurochemical profile (VIP, NPY, reelin, calretinin) characteristic of CGE-lineage interneurons.

{
  "n": null,
  "doi": "10.1093/cercor/bhp310",
  "claim": "Cortical 5-HT3A receptor-expressing interneurons are predominantly generated in the caudal ganglionic eminence; in utero CGE grafts of E14 Htr3a-GFP+ cells reproduce the supragranular laminar distribution and neurochemical profile (VIP, NPY, reelin, calretinin) characteristic of CGE-lineage interneurons.",
  "title": null,
  "cite_key": "Vucurovic2010",
  "evidence": "Body, Results: Tg(Htr3a-GFP) mouse line, in utero grafts of CGE vs AEP/POA explants, immunohistochemistry, and patch-clamp recordings.",
  "effect_size": "In Htr3a-GFP+ NPY cluster: 52% reelin+; in VIP cluster: 23% reelin+",
  "figure_data": [
    {
      "type": "marker_co-expression",
      "unit": "percent",
      "value": 52,
      "metric": "% reelin+ in Htr3a-GFP NPY cluster",
      "source": "Lee 2010 quantification",
      "context": "5HT3aR-GFP+ NPY-cluster CGE-derived interneurons in mouse cortex"
    },
    {
      "type": "marker_co-expression",
      "unit": "percent",
      "value": 23,
      "metric": "% reelin+ in Htr3a-GFP VIP cluster",
      "source": "Lee 2010 quantification",
      "context": "5HT3aR-GFP+ VIP-cluster CGE-derived interneurons in mouse cortex"
    }
  ],
  "text_access": "fulltext",
  "study_system": "mouse cortex, Tg(Htr3a-GFP), in utero CGE grafts",
  "_source_cluster": "cluster_02_development_lineage",
  "replication_status": "replicated",
  "_source_cluster_index": 10,
  "claim_source_sentence": "Various techniques including homochronic in utero grafts performed at the peak of genesis of cortical 5-HT 3A + interneurons revealed that these neurons are mainly generated in the CGE.",
  "replication_evidence_dois": [
    "10.1523/jneurosci.4515-09.2010",
    "10.1523/jneurosci.1869-10.2010"
  ],
  "effect_size_source_sentence": "Interestingly, Reelin was expressed in 52% of cells in the NPY-cluster, whereas only 23% of cells of the VIP-cluster contained this marker."
}

[
  "paper:paper-0336a131683f"
]

[
  {
    "ref": "paper:paper-0336a131683f"
  }
]

{
  "mode": "public_source_pointer_with_short_context",
  "notes": [
    "Local review repositories are read-only inputs.",
    "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
  ],
  "source_commit_sha": "95e761177f7d2ec565983d3307c14ec238f9677c",
  "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewVIP"
}