Details

scope
rat L1 somatosensory cortex acute slices, Patch-seq
section_id
section_05
source_url
https://github.com/AllenNeuralDynamics/ComputationalReviewVIP/blob/95e761177f7d2ec565983d3307c14ec238f9677c/evidence/section_05_evidence_package.json
effect_size
n=67 L1 interneurons profiled with all three modalities; concordance between firing-pattern class and transcriptomic class qualitatively strong
review_repo
ComputationalReviewVIP
section_ref
wiki_page:computationalreviewvip-05-electrophysiology
source_kind
review_finding
source_path
evidence/section_05_evidence_package.json
source_span
In a separate set of experiments, we carried out patch-clamping on an additional set of 67 L1 interneurons in acute cortical slices using the Patch-seq protocol.
study_system
rat L1 somatosensory cortex acute slices, Patch-seq
section_title
Intrinsic Electrophysiology
evidence_summary
rat juvenile somatosensory cortex acute slices; Patch-seq workflow combining whole-cell recordings, morphology recovery, and scRNA-seq; n=67 L1 interneurons
review_bundle_ref
analysis_bundle:ab-2ce40c33e827
replication_status
replicated
review_package_ref
analysis_bundle:ab-2ce40c33e827
source_artifact_ref
wiki_page:computationalreviewvip-05-electrophysiology
origin_url
https://github.com/AllenNeuralDynamics/ComputationalReviewVIP/blob/95e761177f7d2ec565983d3307c14ec238f9677c/evidence/section_05_evidence_package.json
commit_sha
95e761177f7d2ec565983d3307c14ec238f9677c
created_by
persona-jerome-lecoq-gbo-neuroscience
repository_url
https://github.com/AllenNeuralDynamics/ComputationalReviewVIP
Raw fields (5)
claim_text
Patch-seq enables simultaneous extraction of intrinsic electrophysiology, morphology and full transcriptome from single cortical interneurons; in 67 L1 interneurons the joint Patch-seq profile shows strong agreement between firing-pattern-based class (eNGC vs SBC) and transcriptomic class.
raw_fields
{
  "n": 67,
  "doi": "10.1038/nbt.3445",
  "claim": "Patch-seq enables simultaneous extraction of intrinsic electrophysiology, morphology and full transcriptome from single cortical interneurons; in 67 L1 interneurons the joint Patch-seq profile shows strong agreement between firing-pattern-based class (eNGC vs SBC) and transcriptomic class.",
  "title": null,
  "cite_key": "Cadwell2016",
  "evidence": "rat juvenile somatosensory cortex acute slices; Patch-seq workflow combining whole-cell recordings, morphology recovery, and scRNA-seq; n=67 L1 interneurons",
  "effect_size": "n=67 L1 interneurons profiled with all three modalities; concordance between firing-pattern class and transcriptomic class qualitatively strong",
  "text_access": "fulltext",
  "study_system": "rat L1 somatosensory cortex acute slices, Patch-seq",
  "_source_cluster": "cluster_04_intrinsic_electrophysiology",
  "replication_status": "replicated",
  "_source_cluster_index": 9,
  "claim_source_sentence": "In a separate set of experiments, we carried out patch-clamping on an additional set of 67 L1 interneurons in acute cortical slices using the Patch-seq protocol.",
  "replication_evidence_dois": [
    "10.1038/nbt.3443",
    "10.1126/science.adf6484",
    "10.1038/s41586-020-2907-3"
  ],
  "effect_size_source_sentence": "In a separate set of experiments, we carried out patch-clamping on an additional set of 67 L1 interneurons in acute cortical slices using the Patch-seq protocol."
}
source_refs
[
  "paper:paper-1ce98e1d2969"
]
evidence_refs
[
  {
    "ref": "paper:paper-1ce98e1d2969"
  }
]
source_policy
{
  "mode": "public_source_pointer_with_short_context",
  "notes": [
    "Local review repositories are read-only inputs.",
    "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
  ],
  "source_commit_sha": "95e761177f7d2ec565983d3307c14ec238f9677c",
  "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewVIP"
}

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