VIP-expressing 5-HT3aR+ cortical interneurons in mouse S1 are electrically the…

VIP-expressing 5-HT3aR+ cortical interneurons in mouse S1 are electrically the most excitable cortical interneuron group, characterized by very high input resistance, long membrane time constant, prominent sag and low rheobase, with adapting or bursting firing dominating over irregular subtypes; this contrasts sharply with the NPY-cluster (Lamp5/neurogliaform) of 5-HT3aR+ cells.

{
  "n": 22,
  "doi": "10.1093/cercor/bhp310",
  "claim": "VIP-expressing 5-HT3aR+ cortical interneurons in mouse S1 are electrically the most excitable cortical interneuron group, characterized by very high input resistance, long membrane time constant, prominent sag and low rheobase, with adapting or bursting firing dominating over irregular subtypes; this contrasts sharply with the NPY-cluster (Lamp5/neurogliaform) of 5-HT3aR+ cells.",
  "title": null,
  "cite_key": "Vucurovic2010",
  "evidence": "scRT-PCR + whole-cell patch clamp on biocytin-filled 5HT3aR-EGFP+ cortical INs in mouse S1 (Lee 2010 Table 1)",
  "effect_size": "VIP-cluster (n=22): Rin 761.5 ± 306.5 MΩ, τm 40.6 ± 19.5 ms, sag 12.1 ± 7.8%, rheobase 17.7 ± 9.2 pA, RMP −56.0 ± 3.9 mV, 1st-AP duration 0.95 ± 0.37 ms, AP amplitude 83.8 ± 9.8 mV; firing 68% adapting, 32% bursting (n=15/22 vs 7/22). NPY-cluster (n=31): Rin 391.9 ± 124.2 MΩ, τm 25.7 ± 12.1 ms, sag 5.8 ± 6.0%, rheobase 64.8 ± 71.4 pA",
  "text_access": "fulltext",
  "study_system": "mouse S1 cortex 5-HT3aR-EGFP transgenic; whole-cell + scRT-PCR",
  "_source_cluster": "cluster_04_intrinsic_electrophysiology",
  "replication_status": "replicated",
  "_collapsed_provenance": [
    {
      "doi": "10.1093/cercor/bhp310",
      "claim": "In juvenile mouse S1, 5-HT3aR-expressing interneurons partition unsupervised into two clusters: a VIP-cluster (n=22) and an NPY-cluster (n=31). The VIP-cluster occupies layers II–III exclusively (no L1 cells), exhibits bipolar vertically oriented dendrites, and has the highest input resistance and longest membrane time constant of the entire sample — establishing the VIP intrinsic-physiology fingerprint as a high-input-resistance bipolar interneuron with two firing modes.",
      "source_cluster": "cluster_04_intrinsic_electrophysiology",
      "source_cluster_index": 85
    }
  ],
  "_source_cluster_index": 46,
  "claim_source_sentence": "Cells of the VIP-cluster exhibited the highest input resistance and membrane time constant of our sample.",
  "replication_evidence_dois": [
    "10.1093/cercor/bhv202",
    "10.1523/jneurosci.22-17-07389.2002"
  ],
  "effect_size_source_sentence": "Input resistance (MΩ) 761.45 ± 306.46 391.92 ± 124.15 ... Time constant (ms) 40.61 ± 19.53 25.65 ± 12.09 ... Sag index (%) 12.09 ± 7.83 5.84 ± 6.02 ... Rheobase (pA) 17.72 ± 9.22 64.83 ± 71.36"
}

VIP-cluster (n=22): Rin 761.5 ± 306.5 MΩ, τm 40.6 ± 19.5 ms, sag 12.1 ± 7.8%, rheobase 17.7 ± 9.2 pA, RMP −56.0 ± 3.9 mV, 1st-AP duration 0.95 ± 0.37 ms, AP amplitude 83.8 ± 9.8 mV; firing 68% adapting, 32% bursting (n=15/22 vs 7/22). NPY-cluster (n=31): Rin 391.9 ± 124.2 MΩ, τm 25.7 ± 12.1 ms, sag 5.8 ± 6.0%, rheobase 64.8 ± 71.4 pA

[
  "paper:paper-0336a131683f"
]

[
  {
    "ref": "paper:paper-0336a131683f"
  }
]

{
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  "notes": [
    "Local review repositories are read-only inputs.",
    "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
  ],
  "source_commit_sha": "95e761177f7d2ec565983d3307c14ec238f9677c",
  "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewVIP"
}