- claim_text
Channelrhodopsin-2-assisted circuit mapping (CRACM) in mouse brain slices enables sub-cellular resolution mapping of long-range callosal cortico-cortical projections, demonstrating that interhemispheric callosal axons make functional excitatory connections onto identified postsynaptic pyramidal cells in defined cortical layers.
- raw_fields
{
"n": null,
"doi": "10.1038/nn1891",
"claim": "Channelrhodopsin-2-assisted circuit mapping (CRACM) in mouse brain slices enables sub-cellular resolution mapping of long-range callosal cortico-cortical projections, demonstrating that interhemispheric callosal axons make functional excitatory connections onto identified postsynaptic pyramidal cells in defined cortical layers.",
"cite_key": "Petreanu2007",
"evidence": "ChR2 viral expression + brain-slice photostimulation + whole-cell recording of identified pyramidal cells in mouse cortex.",
"effect_size": "CRACM reliably evokes single action potentials in ChR2+ callosal axons and maps their synaptic connections onto postsynaptic neurons in identified layers.",
"text_access": "abstract_only",
"study_system": "Mouse cortical slices; CRACM optogenetic mapping of callosal connections.",
"argument_role": "supporting",
"replication_status": "replicated",
"claim_source_sentence": "We applied this technique, ChR2-assisted circuit mapping (CRACM), to map long-range callosal projections.",
"source_provenance_status": "non_substring_match",
"replication_evidence_dois": [
"10.7554/eLife.100478"
],
"effect_size_source_sentence": "In brain slices from ChR2-expressing mice, photostimulation of ChR2-positive axons can be transduced reliably into single action potentials."
}- source_refs
[
"paper:987379eb-ecdd-42eb-818f-383280a8fca9"
]
- evidence_refs
[
{
"ref": "paper:987379eb-ecdd-42eb-818f-383280a8fca9"
}
]- source_policy
{
"mode": "public_source_pointer_with_short_context",
"notes": [
"Local review repositories are read-only inputs.",
"SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
],
"source_commit_sha": "79ce062d54a924ce05953ec90aa9d26044d2b48f",
"source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence"
}