Subcellular ChR2-assisted circuit mapping (sCRACM) in mouse barrel cortex shows…

Subcellular ChR2-assisted circuit mapping (sCRACM) in mouse barrel cortex shows that callosal/cortico-cortical excitatory inputs onto identified pyramidal neurons target specific dendritic domains distinct from local and thalamocortical inputs, providing direct evidence that cortico-cortical synapses are subcellularly segregated.

{
  "n": null,
  "doi": "10.1038/nature07709",
  "claim": "Subcellular ChR2-assisted circuit mapping (sCRACM) in mouse barrel cortex shows that callosal/cortico-cortical excitatory inputs onto identified pyramidal neurons target specific dendritic domains distinct from local and thalamocortical inputs, providing direct evidence that cortico-cortical synapses are subcellularly segregated.",
  "cite_key": "Petreanu2009",
  "evidence": "ChR2 expression in two thalamic nuclei, whisker motor cortex, and local excitatory neurons in mouse barrel cortex + whole-cell patch + sCRACM mapping.",
  "effect_size": "Long-range cortico-cortical inputs from M1 occupy distinct dendritic compartments from local inputs and thalamocortical inputs on barrel cortex pyramidal cells.",
  "text_access": "abstract_only",
  "study_system": "Mouse barrel cortex; sCRACM circuit mapping.",
  "argument_role": "supporting",
  "replication_status": "replicated",
  "claim_source_sentence": "Here we developed an efficient channelrhodopsin-2 (ChR2)-assisted method to map the spatial distribution of synaptic inputs, defined by presynaptic ChR2 expression, within the dendritic arborizations of recorded neurons.",
  "source_provenance_status": "non_substring_match",
  "replication_evidence_dois": [
    "10.1016/j.neuron.2011.07.029"
  ],
  "effect_size_source_sentence": "The dendrites of individual cortical neurons overlap with numerous types of local and long-range excitatory axons, but axodendritic overlap is not always a good predictor of actual connection strength."
}

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