In mouse V1 layer 2/3, two-photon holographic activation of two 'pattern comple…

In mouse V1 layer 2/3, two-photon holographic activation of two 'pattern completion' neurons within an optogenetically imprinted ensemble can recall the entire ensemble and elicit the corresponding Go/No-Go behavior, even without visual stimulus.

{
  "n": 0,
  "doi": "10.1016/j.cell.2019.05.045",
  "claim": "In mouse V1 layer 2/3, two-photon holographic activation of two 'pattern completion' neurons within an optogenetically imprinted ensemble can recall the entire ensemble and elicit the corresponding Go/No-Go behavior, even without visual stimulus.",
  "cite_key": "CarrilloReid2019",
  "evidence": "Simultaneous two-photon GCaMP6s imaging and C1V1 holographic optogenetics in head-fixed mice performing a contrast Go/No-Go task; ensembles imprinted by repeated visual presentation; subsequent single/dual-cell stimulation tested for ensemble recall and behavioral effect.",
  "effect_size": "2 pattern-completion neurons sufficient to trigger ensemble recall and correct behavioral response",
  "text_access": "fulltext",
  "study_system": "mouse V1 L2/3 in vivo, awake head-fixed, two-photon all-optical, Go/No-Go visual contrast task",
  "argument_role": "supporting",
  "replication_status": "independently_replicated",
  "claim_source_sentence": "Finally, optogenetic targeting of only two behaviorally relevant pattern completion neurons could trigger behavior, even in the absence of visual stimulus, as long as the behaviorally relevant ensemble could be recalled by their activation.",
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  "replication_evidence_dois": [
    "10.1038/s41593-025-02055-5",
    "10.1038/s41593-025-02024-y"
  ],
  "effect_size_source_sentence": "The activation of a small set of pattern completion neurons via two-photon holographic optogenetics triggers neuronal ensembles that appear to be necessary and sufficient for behavior."
}

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Simultaneous two-photon GCaMP6s imaging and C1V1 holographic optogenetics in head-fixed mice performing a contrast Go/No-Go task; ensembles imprinted by repeated visual presentation; subsequent single/dual-cell stimulation tested for ensemble recall and behavioral effect.