- claim_text
Combining non-unity-magnification remote focusing with adaptive optics, dual-plane three-photon microscopy in mouse neocortex achieves single-cell-resolution simultaneous imaging from two temporally multiplexed planes separated by up to 600 µm — supporting concurrent acquisition of genetically defined cell types in any pair of cortical layers across the column.
- raw_fields
{
"n": 0,
"doi": "10.1364/boe.544383",
"claim": "Combining non-unity-magnification remote focusing with adaptive optics, dual-plane three-photon microscopy in mouse neocortex achieves single-cell-resolution simultaneous imaging from two temporally multiplexed planes separated by up to 600 µm — supporting concurrent acquisition of genetically defined cell types in any pair of cortical layers across the column.",
"cite_key": "Cloves2024",
"evidence": "Instrument paper combining 3P + AO + dual-plane multiplexing; demonstrated cross-layer simultaneous imaging.",
"effect_size": "dual-plane 3P imaging up to 600 µm axial separation, single-cell resolution",
"text_access": "fulltext",
"study_system": "Mouse neocortex (3P microscope instrument paper)",
"argument_role": "supporting",
"replication_status": "instrument-paper",
"claim_source_sentence": "Here, we combine non-unity magnification remote focusing with adaptive optics to achieve single-cell resolution imaging from two temporally multiplexed planes separated by up to 600 µm. This approach enables the simultaneous acquisition of neuronal activity from genetically defined cell types in any pair of cortical layers across the mouse neocortical column.",
"source_provenance_status": "ok",
"replication_evidence_dois": [],
"effect_size_source_sentence": "achieve single-cell resolution imaging from two temporally multiplexed planes separated by up to 600 µm."
}- source_refs
[
"paper:paper-307945efdb32"
]
- source_span
Here, we combine non-unity magnification remote focusing with adaptive optics to achieve single-cell resolution imaging from two temporally multiplexed planes separated by up to 600 µm. This approach enables the simultaneous acquisition of neuronal activity from genetically defined cell types in any pair of cortical layers across the mouse neocortical column.
- evidence_refs
[
{
"ref": "paper:paper-307945efdb32"
}
]- source_policy
{
"mode": "public_source_pointer_with_short_context",
"notes": [
"Local review repositories are read-only inputs.",
"SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
],
"source_commit_sha": "79ce062d54a924ce05953ec90aa9d26044d2b48f",
"source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence"
}